ماستر (LMD)
French
2017
Cholinestérase et toxicité des extraits aqueux foliaires de Peganum Harmala sur Drosophile Melanogaster dans la région de GHARDAIA
امل بن ضاحو, عائشة حميد
كلية علوم الطبيعة والحياة وعلوم الأرض-جامعة غرداية · الجزائر
This study investigates the toxic effects of aqueous foliar extracts of Peganumharmala L on the activity of the cholinesterase enzyme in adult Drosophila melanogaster inthe Sebseb region of Ghardaïa. P. Harmala leaves are harvested at Hay ELJADR, andsubjected to drying, grinding and reflux extraction. Toxicity tests are carried out at differentdoses of the aqueous extract of P.harmala L, treatment in vivo, either by contact (contacttoxicity and inhalation) or by consumption of food mixed with the leaf extract (toxicity byingestion ), Determines physiological parameters (lethal time 50 or LD50, mortality rate anddose 50 or LD50) and the in vitro treatment makes it possible to determine biochemicalparameters (cholinesterase activity, activity The in vivo treatment revealed a percentage ofmortality which increased as a function of the extract concentration applied: 55.55% forcontact treatment at the dose 80% of applied extract and 100% for treatment with Ingestion ata dose of 80%. The lethal time 50 (TL50) registers 8.80 hours with a lethal dose of 0.03mg /ml per contact, 33.7 hours with a dose of 0.005mg / ml per Treatment, toxicity of contacttreatment seems to be the most effective.However, treatment of Drosophila individuals invitro with different doses of P.harmala extract shows a significant decrease in the moreperceptible cholinesterase activity at the highest dose, 0.03 ± 0.02 nanomole / min / ml for the100% , In comparison with the cholinesterase activity in the control individuals, which isequal to 3.39 ± 1.24 nanomole / min / ml, thus showing a decrease in the level of proteins forall batches treated, the maximum decrease being noted at A pure dose, with 10.5 ± 6.71μg /ml, similarly a decrease in the specific activity is noted, the maximum decrease is at the puredose with 0.068 ± 0.048 nanomole / mn / μg. The aqueous foliar extract of P. Harmala hasinsecticidal and anticholinesterasic properties in D. Melanogaster.