Cholinestérase et toxicité des extraits aqueux foliaires de Peganum Harmala sur Drosophile Melanogaster dans la région de GHARDAIA

الموضوعات

علوم بحتة وطبيعية

الملخص

This study investigates the toxic effects of aqueous foliar extracts of Peganumharmala L on the activity of the cholinesterase enzyme in adult Drosophila melanogaster inthe Sebseb region of Ghardaïa. P. Harmala leaves are harvested at Hay ELJADR, andsubjected to drying, grinding and reflux extraction. Toxicity tests are carried out at differentdoses of the aqueous extract of P.harmala L, treatment in vivo, either by contact (contacttoxicity and inhalation) or by consumption of food mixed with the leaf extract (toxicity byingestion ), Determines physiological parameters (lethal time 50 or LD50, mortality rate anddose 50 or LD50) and the in vitro treatment makes it possible to determine biochemicalparameters (cholinesterase activity, activity The in vivo treatment revealed a percentage ofmortality which increased as a function of the extract concentration applied: 55.55% forcontact treatment at the dose 80% of applied extract and 100% for treatment with Ingestion ata dose of 80%. The lethal time 50 (TL50) registers 8.80 hours with a lethal dose of 0.03mg /ml per contact, 33.7 hours with a dose of 0.005mg / ml per Treatment, toxicity of contacttreatment seems to be the most effective.However, treatment of Drosophila individuals invitro with different doses of P.harmala extract shows a significant decrease in the moreperceptible cholinesterase activity at the highest dose, 0.03 ± 0.02 nanomole / min / ml for the100% , In comparison with the cholinesterase activity in the control individuals, which isequal to 3.39 ± 1.24 nanomole / min / ml, thus showing a decrease in the level of proteins forall batches treated, the maximum decrease being noted at A pure dose, with 10.5 ± 6.71μg /ml, similarly a decrease in the specific activity is noted, the maximum decrease is at the puredose with 0.068 ± 0.048 nanomole / mn / μg. The aqueous foliar extract of P. Harmala hasinsecticidal and anticholinesterasic properties in D. Melanogaster.

روابط وملفات

التعريف والنوع

رقم الوثيقة

f5bd8f01-1859-4bc6-9905-5de7bca7830e

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

ماستر (LMD)

نوع الملف

pdf text

أسماء الملفات

955831_1.pdf

بيانات النشر

ألقاب المؤلفين

[{"name_ar":"امل بن ضاحو","title_ar":"اعداد","title_en":"Preparation"},{"name_ar":"عائشة حميد","title_ar":"اشراف","title_en":"Supervision"}]

اللغة

French

المصدر والدورية

اسم المصدر

Cholinestérase et toxicité des extraits aqueux foliaires de Peganum Harmala sur Drosophile Melanogaster dans la région de GHARDAIA

المحتوى والصفحات

عدد الصفحات

0

كلمات الباحثين

Cholinestérase, Mortalité, Drosophile Melanogaster, Peganum Harmala, extraits foliaires, GHARDAÏA Cholinesterase, Mortality, Drosophila Melanogaster, Peganum Harmala, leaf extracts, GHARDAÏA.

إشراف وإعداد

الإشراف

عائشة حميد

الإعداد

امل بن ضاحو

الاقتباسات الببليوغرافية