Serological Detection and Molecular Characterization of Cytomegalovirus and its Glycoprotein B (UL55) among Sudanese Renal Transplant Recipients
Hind Haidar Ahmed AbdAlla, Al-Fadhil Al-Obeid Omer
كلية الدراسات العليا-جامعة السودان للعلوم والتكنولوجيا · السودان
This is analytical cross sectional study conducted at Kidney transplanted association hospital and Ahmed Gassim teaching hospital in Khartoum state in the period from 2013 to 2015, was aimed to detect CMV using ELISA and quantitative real-time PCR (qRT PCR), estimate viral loads and to determine the distribution of gB genotypes among Sudanese renal transplant recipients by DNA sequencing.One hundred and four renal transplant recipients were included in this study. Blood and serum samples were collected from them, their age ranged from 11 to 72 years with mean age of 37 years. Males were 72(69.2%), while females were 32(30.8%). In this study, 50(48%) of them received their organs in local hospitals, while 54(52%) received their organs in abroad. Most of the renal transplant recipients’ received organs from relative donors 79(76%) and only 25 (24%) received organs from non-relative donors. The mean post-transplantation duration was 54 months, ranged from 0.05) with sex and types of immunosuppressive therapy received by transplant recipients, while there is a significant difference (P.value = 0.05) between high viral loads and types of immunosuppressive therapy received by transplant recipients. At the same time the correlation between high viral loads (>1000 copies /ml) and development of CMV disease observed significant difference (P.value = 0.00), in which, 14/17 (82%) of patients had clinical symptoms of CMV disease with high viral loads and 22/23 (95.7%) of patients had no clini¬cal symptoms of CMV disease with low viral loads (<1000 copies/ml). The most common presenting symptoms of CMV disease were fever, fever and leucopenia, and gastrointestinal disease. The distribution of gB genotypes in Sudanese renal transplant recipients observed that gb3 was the most frequent genotype (80%) while gB4 was (20%) and no mixed genotypes were detected.In conclusion, qRT-PCR from plasma samples is very sensitive for detection of CMV replication and was more sensitive than ELISA technique in detecting CMV in renal transplant patients. Viral loads were lower with asymptomatic patients. CMV gB3 was considered the most predominant glycoprotein B genotype in Sudanese renal transplant recipients with CMV disease.