رسائل ماجيستير
English
2014
Phylogenetic Relationship among Closely
Related Species of the Genera Lens, Vicia,
Lathyrus and Pisum (Leguminosae) in
Palestine
غالب عدوان, غدير عمر, مرام محمد فياض صقر
كلية الدراسات العليا-جامعة النجاح الوطنية · فلسطين
Leguminosae or Fabaceae is the third largest flowering plant family, which
is an important economically for food production and soil fertility. Four
Palestinian genera of the Fabaceae family which are Lens, Vicia, Lathyrus
and Pisum have overlapping morphological characteristics among them
were the subject of this study. Different molecular techniques are used to
determine the relationship and genetic diversity among Leguminosae
species of interest including internal transcribed spacers (ITS) sequencing.
This study was performed to identify the phylogenetic relationship among
9 closely related species belong to 4 genera of Leguminosae family: Lens
culinaris, Vicia sativa, Vicia palaestina, Vicia peregrina, Vicia faba, Vicia
narbonensis, Lathyrus aphaca, Pisum fulvum and Pisum sativum.
Moreover, In spite of that L. culinaris and V. Palaestina are two species
belong to different genera; their morphological features are similar to a
high degree. Therefore, this study was performed to determine the
taxonomic classification of V. palaestina and its phylogenetic relationship
to the morphological closely related species L. culinaris.
Morphological classification was conducted for some closely related
species of the Leguminosae family in Palestine. The studied plant species
were collected from natural wild habitat. Then a representative plant
xii
specimen of each species was deposited at An-Najah National University
Herbarium. Then molecular analysis was determined using 18S and 28S as
universal primers for amplifying and sequencing the ITS region.
Evolutionary divergence between sequences was estimated and
phylogenetic tree was constructed using the Kimura 2-parameter model and
Unweighted Pair Group method, respectively.
Each sample ITS sequence of the examined species as well as Salvia
dominica, as an out group, were introduced into the GenBank. The
phylogenetic analysis among studied species revealed that, V. palaestina
(KJ864941) and P. sativum (KJ864946) have a high average of intraspecies
genetic divergence (8.95%). While, low average of intraspecies genetic
divergence (1.81%) between V. faba (KJ864957, KJ864958) and V.
peregrina (KJ864954) was recorded. Moreover, V. peregrina, V. faba, V.
narbonensis, V. palaestina, L. culinaris, and V. sativa were grouped into
one clade (clade І). However, V. sativa occupied the farther position in
clade І and separated from other species of Vicia genus. While, P. sativum,
P. fulvum and L. aphaca were grouped into the same clade (clade ІІ). On
the other hand, L. culinaris formed a cluster within the core of Vicia near to
V. Palaestina.
In this study, phylogenetic relationship among the closely related species
under study of Leguminosae family was detected accurately using ITS
sequencing. Also this technique revealed the close relationship between L.
culinaris and V. palaestina, as the two species are related to the same genus
instead of two different genera