Some Morphometric Studies on Spermatozoa Stained with Different Cytological Stains in Gezira Fertility Center, Wad Medani, Sudan (2017)
آلاء مبارك أحمد البشير, البدوي عبدالباقي طلحة, عبدالرحيم علي بابكر
كلية علوم المختبرات الطبية-جامعة الجزيرة · السودان
Recentalarmingtrends ofasubstantialriseinthenumberofcases ofinfertilityandsubfertility,with asmany as30-40%beingattributedtomale-factorassociatedcauses,havecreatedaneed forfurtherstudiesandadvancementsinsemen analysis.Despitethefocusonsemen analysis overlast decates,assessmentofspermmorphologyhas notbeengivendueimportance,althoughit is a simple,standardandbaselinediagnosticmodality.Thisisacross sectional,comparative laboratorybasedstudy,aimedtodetectebetterstainfordiagnosis ofspermmorphology,this studyconductedinGaziraFertilityCenter fromtheperiodfromJanruary 2017toApril2017,320 semensamplescollected from 80 patientsattending to the center suffering from subfirtility,theiragerangeform (20–45)years. Prepared semen films,thesamplewerestainedbyPapanicolau,Giemsa, H&Eand Diffquickstain.Interpretationof resultbylightmicroscope.TheresultfoundthePap staingiveexcellentandclearstaintospermatozoaafollowedbyGiemsa,H&E,Diffquick stainrespectively.ThisstudyconcludedthemostefficientdyeisPapanicolaustain followedby giemsastainforhead, neck,andtailmorphololgyofsperm.Thisstudyrecommendedtoused stainedsemensmearreplacedbywetpreparsmearandandusingof papstainandgiemsastain fordiagnosis ofspermmorphology.Also its simple and givedstanderddiagnostic assessment.