رسائل دكتوراة
English
2016
Isolation and identification of some biologically active compounds from some Sudanese medicinal plants
Malik A. Abddrahman, Ahmed Elsadig Mohammed Saeed, Missa Mohammed Saleh Abdealsiede
كلية الدراسات العليا-جامعة السودان للعلوم والتكنولوجيا · السودان
Chrozophora Plicata (argassi), Senna Singueana (Umm saaba) andStylochiton borumensis (Moura) plants are widely used in traditional medicine totreat bacterial, fungal and viral infections. As a result there was need to investigateextracts of these plants and to provide scientific proof for their wide application intraditional medicine system .Extraction of leaves and seeds of C. Plicata, S. Singueana and leaves of S.borumensis using solvents of increasing polarity, namely, petroleum ether,chloroform, ethyl acetate and methanol.For qualititative and quantitative estimation of the secondary metabolitescompounds of this plants and found that they contain flavonoids, tannins, alkaloidsand glycosides in different concentration of the plants.The steroid was extracted from chloroform extracts of leaves and seeds ofthe plants and identified by GC-MS.The fixed oil was extracted using petroleum ether and identified the fattyacids using gas chromatography mass spectrometer.Ethyl acetate and methanol extracts for all plants of this study showed highantioxidant scavenging activity using DPPH method.S. borumensis leaves contained high concentration of essential and nonessential amino acids, S. Singueana and C. Plicata leaves contained highconcentration ranges of alanine, glutamic and leucine: 754.7-633.7 ppm, 835.9-922.4 ppm, and 731.6-728.6 ppm, respectively.The percentages of total forms of Pb, Cu and As, in the reference plant materialwere found to be 5.28%, 0.25% and 1.51% respectively; meanwhile toxicconcentrations of heavy metals in the examined plants material were not detectedwhen compared with reference material.The extracts were tested for anti-fungal and anti-bacterial activities, Usingdisc diffusion method, by used two gram positive bacteria (Bacillus subtilis, S.aureus,), two gram negative bacteria (Pseudomonas aeruginosa, Escherichia coli)and two unit-fungal (C. albicans, A. niger), it was demonstrated that extracts of theleaves and seeds of C. Plicata had high anti-bacterial activities against testedorganism and low anti-fungal activity against Candida albicans but all extracts ofleaves and seeds of the plants showed high activity against A. niger.S. Borumensis leaves showed that the petroleum ether and chloroformextracts were not active against Staphylococcus aureus, Pseudomonas aeruginosabut the extracts showed high antifungal activity.All seeds and leaves extracts of the plants under study displayed moderatetoxicity against Brine Shrimps lethality. All extracts of C. Plicata leaves and seedswere non toxic against vero cell lines suggesting that they were safe. All leaves andseeds extracts of C. Plicata were active against G. lambelia; chloroform andmethanol extracts for leaves and seeds respectively, were the most effectivecompared to that exhibited by metronidazole drug (89%).Two new compounds were isolated from methanol extract of the leaves ofChrozophora plicata. The new compounds were elucidated by (MS) and (NMR):1- Kaempferol -3-O-C1″ glucose2- Methyl 2-[3-hydroxy-5-(propan-2-yl)phenyl]butanoateC. Plicata, S. Singueana and S. borumensis revealed variation in variousbiochemicals analysed; their seeds and leaves could be helpful for pharmaceutical,antimicrobial and antioxidants agents as well as food industries
