رسائل دكتوراة
English
2021
Chemical Characterization and Biological Activity of Oils Extracted from Some Medicinal Plants Grown in Suda
خالد الرياح الامين, محمد عبدالكريم محمد
كلية الدراسات العليا-جامعة السودان للعلوم والتكنولوجيا · السودان
This study was designed to reveal the constituents of the fixed oils (extractedby solvent extraction method) of some Sudanese medicinal plants by usingGC-MS (gas chromatography mass spectrometry), and to assess theirantibacterial , antifungal activity using Disc diffusion method. The chosenplants for this study were: gynandropis gynandra – capparaceae; hibiscusasper – malvaceae; Cucumis prophetarum – cucuribaceae; Citrulluslanatus sub sp. Lanatus cucurbitaceae; and Merremia (Ipomea) Dissecta.The GC-MS analysis of Gynandropis Gynandra oil revealed the presence of17 components and the major constituents were : 9,12-octadecadienoic acid(Z,Z)- methyl ester(19.78%); Linoleic acid ethyl ester (12.16%);9,12-octadecadienoic acid, ethyl ester(11.88%) ; 9-Octadecenoic acid (Z)-methyl ester(11.43%); and Oleic acid(11.35). At a concentration of100mg/mL the oil was screened for antimicrobial activities using four stander bacterial strains to assess the antimicrobial activity against: - Gram +ve bacteria; Staphylococcus aureus-Gram +ve ;Escherichia coli - Gram +ve bacteria and Pseudomonas aeruginosa-Gram -ve bacteria, in addition the oil was screened for activity but unfortunately the results indelicate that the fixed oil of come has no any activities against these microorganisms. The seed oil showed 20components dominated by: 9,12-Octadecadienoic acid (Z,Z) - methyl ester(46.32%); Methyl stearate (18.39%); Hexadecanoic acid, methyl ester(18.10%) and 9-Octadecenoic acid (Z)- methyl ester(13.22%). Ata concentration of 100mg/mL the oil was screened for previous antibacterial and antifungal where the results were shown a moderate antibacterial activity against Escherichia coli and active response against activity. The GC-MS analysis of Merremia (Ipomea) Dissect seed oil showed 20 components dominated by: 9,12-Octadecadienoic acid (Z,Z)-, methyl ester (32.78%); Hexadecanoic acid, methyl ester (23.04%); 9-Octadecenoic acid (Z)-, methyl ester(22.04%) and methyl stearate (13.12%). The oil showed moderate antibacterial activity against Bacillus subtilise, but showed no . The GC-MS analysis of Curcumin prophet arum revealed the presence of 9 components, and the major constituents were: Linoleic acidethyl ester (54.25%); 9,12-Octadecadienoic acid (Z,Z)-, methyl ester(16.86%); gamma.-Ergosterol (8.14%); and n-Hexadecanoic acid (6.06%). The fixed oil did not show any activities against the previous microorganisms.