Development and Validation of New Colorimetric Method for the Analysis of Propanolol Hydrochloride in Bulk and Tablet Dosage Form

الموضوعات

طب

الملخص

AbstractBackground:The pharmacological and therapeutic relevance of propanolol hydrochloride (PPH) have lead to development of several methods for its analysis. Colorimetric method, particularly which is based on cerium (IV) sulfate spectrophtometry, has been established as being very sensitive for PPH analysis.Objective:The objective of this study was to develop and validate a new colorimetric method based on ceric (IV) sulfate for the analysis of PPH.Method:The proposed method is an indirect spectrophotometric one based on the ability of cerium (IV) sulfate to oxidize PPH and to bleach the colour of the indicator methyl orange. In this method, PPH reacted with a measured excess of cerium (IV) sulphate in acidic medium and the unreacted oxidant was determined by reacting with methyl orange (MO) followed by absorbance measurement at 508 nm.Results:The optimized method obeys Beer's Lamber law over the concentration range (0.5-3.5) μg/ml. The correlation coefficient (R2) was 0.99983 and 0.999 for the standard and sample calibration curve respectively. The detection and quantitation limit were found to be (0.0899 and 0.2725) μg/ml respectively. Moreover, the values of RSD% was found to be smaller than 2 and that of (t) was found to be smaller than the tabulated one (4.30). On the other hand the solution color was found to be stable for more than two hours.XVIConclusion:A new applicable method for the determination of PPH has been developed and validated as per the current ICH guidelines. The proposed method is simple, rapid and based on the measurement of stable colored species using ceric ammonium sulfate solution as a quantitative reagent.The proposed method does not take more than 15–20 min and is among the most sensitive reported for PPH. An additional advantage of the method is that the absorbance is measured at longer wavelengths where the interference from excipients is far less than at shorter wavelengths.Recommendation:It is recommended to conduct further optimization of the spectrophotomeric technique that involve ceric (IV) sulfate as an oxidizing reagent by utilizing experimental design.Ceric (IV) sulfate must be further investigated with respect to its stability and the effect of heat on the reactions in which it takes part as a reagent so that optimization of the experimental conditions can be based on concrete experimental evidence rather than relying on trial and error.The method is recommended for routine use in pharmaceutical laboratories as a part of industrial quality control.

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التعريف والنوع

رقم الوثيقة

fa3e3a6c-64d4-43d3-8c01-57499878cbe5

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل ماجيستير

نوع الملف

pdf text

أسماء الملفات

51814_1.pdf

بيانات النشر

ألقاب المؤلفين

[{"name_ar":"رشيد أحمد جادكريم","title_ar":"اشراف","title_en":"Supervision"},{"name_ar":"سمر علي عبد الرازق علي","title_ar":"اعداد","title_en":"Preparation"}]

اللغة

English

المصدر والدورية

اسم المصدر

Development and Validation of New Colorimetric Method for the Analysis of Propanolol Hydrochloride in Bulk and Tablet Dosage Form

المحتوى والصفحات

عدد الصفحات

0

إشراف وإعداد

الإشراف

رشيد أحمد جادكريم

الإعداد

سمر علي عبد الرازق علي

الاقتباسات الببليوغرافية