Determination of Antioxidant Flavonoids in Sudanese Honey Samples of Different Regional Sources by Solid Phase Extraction and High Performance Liquid Chromatography

الموضوعات

علوم بحتة وطبيعية

الملخص

In this study, flavonoids were extracted by solid phase extraction(SPE) from seven floral honey samples, of different botanical originfrom different regions of Sudan. The flavonoids were determined byhigh performance liquid chromatography (HPLC) technique usingphoto diode array detector (PDA).An isocratic and gradient systems for the resolution, identification andquantification of five flavonoids, namely: quercetin, kaempferol,apigenin, hesperetin and isorhamnetin, were developed. Although theisocratic system resolved the five compounds, however it sufferedfrom interference by the complex mixture of honey samples. Thegradient system resolved three of five flavonoids, namely: (quercetin,kaempferol, and isorhamnetin), without interference by the complexhoney matrix. Two flavonoids, apigenin and hesperetin, wereobserved to elute at close retention times, which lead to theirinterference with each other when injected in a mixture; however,absorption wavelength selection was found indicative of the presenceor absence of either compound. The quantification of these flavonoidswas done through the calibration curves of their standards. Theobtained results were compared with published results.Some physicochemical properties of honey samples weredetermined.

روابط وملفات

التعريف والنوع

رقم الوثيقة

f221188e-6d50-4cd6-a2fc-0b6646bba85d

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل ماجيستير

نوع الملف

pdf text

أسماء الملفات

62984_1.pdf

بيانات النشر

ألقاب المؤلفين

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اللغة

English

المصدر والدورية

اسم المصدر

Determination of Antioxidant Flavonoids in Sudanese Honey Samples of Different Regional Sources by Solid Phase Extraction and High Performance Liquid Chromatography

المحتوى والصفحات

عدد الصفحات

0

إشراف وإعداد

الإشراف

الرشيد أحمد جاد كريم, سعد محمد حسين إيوب

الإعداد

سوزان زين العابدين مكاوي

الاقتباسات الببليوغرافية