Biological Activity of Black Cumin (Bunium bulbocastarum L.) Seed Extracts Against Tumor Caused by Agrobacterium tumefaciens "SDB0012" Using Potato Disc Bioassay

الموضوعات

علوم تطبيقية وتكنولوجية

الملخص

Black cumin (nigella sativa L.), family Ranunculaceae, is a widely distributed annual herbaceous plant, Nigella sativa has been used as traditional medicine for centuries. The present study was investigating the biological activity of the black cumin oil extracts and essential oil against the tumor using potato disc bioassay. Samples of black cumin seeds were obtained from the local market in Wad Medani City, Gezira State, Sudan. Five cumin products were used; the cumin essential oil which was extracted by distillation, the cumin oil which was extracted by cold extract (using petroleum ether), methanol and ethyl acetate extracts, in addition to a commercial sample of cumin oil which was brought from the local market of Wad Medani City. The indigenous strain of Agrobacterium tumefaciens "SDB0012" was brought from University of Gezira. At first, the oils and their extracts were tested against A. tumefaciens using potato disc bioassay. The results showed that, ethyl acetate extract inhibited the bacterium as 50%, meanwhile, 10% inhibition zone was observed for petroleum ether extract and the commercial oil sample and 0% inhibition was recorded for methanol extract and essential oil. Any extracts have anti bacterial effect ( including ethyl acetate, Petroleum ether and Commercial sample) did not used in anti tumor activity. methanol and essential oil extracts were used as anti tumor effect and showed the highest inhibition percentage of 100% and 87% respectively. These records was obtained after fourteen day of incubation. After 21 days, methanol extract gave 100% inhibition followed by essential oil (87%). Data was collected based on inhibition in tumor development (zone) caused by A. tumefaciens, using lugols ,reagent which turns tumerigenic tissues into dark brown color. Agrobacterium infection led to development of tumerigenic tissues on the surface of the potato discs 2 cm in rise, as comparing with the discs used as control (complete coverage of the disc surface). It was concluded that methanol extract of black seed was very effective in tumor inhibition and hence, it can be recommended to further studies in this area.

روابط وملفات

التعريف والنوع

رقم الوثيقة

0bd3ee00-9979-4348-8904-8b41c35db0c2

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل ماجيستير

نوع الملف

pdf text

أسماء الملفات

73278_1.pdf

بيانات النشر

ألقاب المؤلفين

[{"name_ar":" Mutamen Ali Abdelgader Kehail ","title_ar":"اشراف","title_en":"Supervision"},{"name_ar":"Magdi Siddig Elhaj Moneir","title_ar":"اعداد","title_en":"Preparation"},{"name_ar":"Mohamed Taha Yousif ","title_ar":"اشراف","title_en":"Supervision"}]

اللغة

English

المصدر والدورية

اسم المصدر

Biological Activity of Black Cumin (Bunium bulbocastarum L.) Seed Extracts Against Tumor Caused by Agrobacterium tumefaciens "SDB0012" Using Potato Disc Bioassay

المحتوى والصفحات

عدد الصفحات

0

إشراف وإعداد

الإشراف

Mutamen Ali Abdelgader Kehail , Mohamed Taha Yousif

الإعداد

Magdi Siddig Elhaj Moneir

الاقتباسات الببليوغرافية