Biological and Biochemical Properties of Two KDM1A Associated Alternatively Spliced SWIRM Domains

الموضوعات

علوم بحتة وطبيعية

الملخص

LSD1 is the first described histone demethylase which demethylatesH3K4me1/2 (Shi et el., 2004), thus, causing transcriptional repression.Alternatively, LSD1 was demonstrated to have H3K9me1/2 demethylase activitywhen bound by androgen receptor, hence, causing transcriptional activation(Schule et al., 2005). LSD1 is commonly recruited by the so called CoREST corecomplex including: RCOR1, HDAC1 and HDAC2 among others and therefore iscoupled with histone deacetylation and transcriptional repression (Foster et al.,2010). It is an important regulator of pluripotency in early development and itoccupies, along with pluripotency factors NANOG and OCT4, the promoters ofmajor lineage determining genes that are poised for activation in the pluripotentstate, (Adamo et al., 2011). There are four described isoforms for LSD1: LSD1,LSD1-E2a, LSD1-8a and LSD1-E2a/E8a (Zibetti et al., 2010). While the Cterminusof LSD1 is extensively studied and the function of the isoforms LSD1-E8aand LSD1-E8aE2a is described, there is scarce knowledge on LSD1 N-terminusunstructured region and the SWIRM domain. In this project I examined the role ofthe differently spliced exon 2a on the function of the SWIRM domain throughgeneration of eight constructs coding for the N-terminal portion of LSD1 SV1 andSV2 fused with a C- or N-terminus FLAG tag. I then performed animmunoprecipitation experiment followed by mass spectrometry and proteomicsanalysis that led to the identification of previously unknown binding partners to theLSD1 SWIRM domain: NONO and IGF2B3.

روابط وملفات

التعريف والنوع

رقم الوثيقة

0b371636-bd1f-4b93-9241-7defd0cc0320

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل ماجيستير

نوع الملف

pdf text

أسماء الملفات

1698874_1.pdf

بيانات النشر

ألقاب المؤلفين

[{"name_ar":"أنطونيو أدامو","title_ar":"اشراف","title_en":"Supervision"},{"name_ar":"يارا فضيلي","title_ar":"اعداد","title_en":"Preparation"}]

اللغة

English

المصدر والدورية

اسم المصدر

Biological and Biochemical Properties of Two KDM1A Associated Alternatively Spliced SWIRM Domains

المحتوى والصفحات

عدد الصفحات

0

كلمات الباحثين

KDM1A , Pluripotency , Splicing Variant , SWIRM domain

إشراف وإعداد

الإشراف

أنطونيو أدامو

الإعداد

يارا فضيلي

الاقتباسات الببليوغرافية