The Role of Glycyrrhiza glabra Root Aqueous Extract in Modulating the Genotoxic Effects of Methotrexate in Albino Male Mice and Acute Lymphoblastic Leukaemia Patients

الموضوعات

طب

الملخص

The present study was carried out to shed light on the chemical analysis of Glycyrrhiza glabra for different active compounds, and on the cytogenetic effects of methotrexate(MTX) and aqueous extract of G. glabra in laboratory mice  (in vivo) and human blood lymphocytes (in vitro). The study also aimed to investigate the role of G. glabra aqueous extract in reducing the cytogenetic effects of MTX in mouse through the parameters mitotic index, and chromosomal aberration in bone marrow, the total and differential counts of leukocytes, and sperm head and tail morphology. The cytogenetic analyses were extended to include blast index, mitotic index, replicative index, sister chromatid exchanges and chromosomal aberrations in human blood lymphocytes in vitro as a result of G. glabra aqueous extract and MTX treatment. the cytogenetic effects of the drug and plant extract were investigated after five days of treatment in mice with three doses for each. For MTX, the doses were 1.2, 1.6, and 2 mg/kg, while the doses of G. glabra   were 400, 600, and 800 mg/kg. Using human blood lymphocyte (72hour) culture, the tested concentrations of MTX were 0.4, 2, and 4 µg/ml, and for G. glabra aqueous extract were 50, 100, and 200 µg/ml. An interaction between plant extract and MTX was carried out through two types of treatments (before, and after MTX treatment) to determine the activity of G. glabra aqueous extract in reducing the drug side effects in vivo .

روابط وملفات

التعريف والنوع

رقم الوثيقة

05bdb477-16c7-4846-a4d9-917dfd44c0cc

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل ماجيستير

نوع الملف

pdf text

أسماء الملفات

14856_1.pdf

بيانات النشر

ترجمة العنوان

دراسة دور المستخلص المائي للجذور عرق السوس في تعديل التأثيرات السمية الوراثية لعقار الميثوتركسيت في ذكور الفأران البيض ومرضى ابيضاض الدم اللمفي الحاد

ألقاب المؤلفين

[{"name_ar":" Esmail K. Shubber","title_ar":"اشراف","title_en":"Supervision"},{"name_ar":"Khulood W. Al-Samarae ","title_ar":"اشراف","title_en":"Supervision"},{"name_ar":"Worood Kamil Al-Malikey","title_ar":"اعداد","title_en":"Preparation"}]

اللغة

English

المصدر والدورية

اسم المصدر

The Role of Glycyrrhiza glabra Root Aqueous Extract in Modulating the Genotoxic Effects of Methotrexate in Albino Male Mice and Acute Lymphoblastic Leukaemia Patients

المحتوى والصفحات

عدد الصفحات

0

إشراف وإعداد

الإشراف

Esmail K. Shubber, Khulood W. Al-Samarae

الإعداد

Worood Kamil Al-Malikey

الاقتباسات الببليوغرافية