Effect of Antiasthmatic Drugs on Cu-Zn Superoxide Dismutase and other Parameters in Hilla City

الموضوعات

علوم تطبيقية وتكنولوجية

الملخص

The study was conducted on asthmatic patients who were treated with inhaled salbutamol [patients (n=41), males=21, females=20] and asthmatic patients who were treated with Montelukast drug [patients (n=37), males=19, females=18], who were reviewing the center of allergies and asthma in Hilla city, for the period from January 2013 to the first thirty-June 2013. Dr. Raad Faanwkh and Dr. Abeer Al-Khfaagy participated in the selection of patients, and healthy subjects [control (n=39), males=19, females=20]. Serum was used to assay antioxidant enzyme activities, while erythrocytes (packed cells) were used to separate and purify the Cu-Zn superoxide dismutase enzyme. The results of the study are as follows: The study showed a significant decrease (P<0.001) in the concentration of total antioxidant capacity (TAC), and Cu-Zn superoxide dismutase activity in the sera of patients with asthma who used inhaled salbutamol or montelukast treatment compared with healthy controls. There was no difference in total antioxidant capacity (TAC) and the enzyme Cu-Zn SODs in the sera of asthma patients who used inhaled salbutamol compared with asthma patients who used the treatment of Montelukast. Significant decrease in the SOD activity and TAC in the sera of patients with asthma (P<0.001) compared with the healthy group. The purified SOD had a specific activity of 2800 U/mg of protein (healthy), 2431.45 U/mg protein for inhaled salbutamol patients, and 2229.2 U/mg protein for treatment with montelukast drug patients, and gave a single band on polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate (SDS). Each of its subunits had a molecular weight of about [(18500 Daltons, control), (18300 Daltons, asthmatic patients)]. Serum levels of Zn, Cu, and Se were significantly different (p<0.001 for all) in patients when compared with healthy subjects. There was a significant decrease (p<0.001) in copper and selenium concentrations and a significant increase (p>0.05) in zinc concentration in the sera of asthmatic patients who used inhaled salbutamol and Montelukast when compared to those of the control group. Highly positive correlation (significant) between Cu and Cu/Zn (r=0.72), p value at the 0.01 level (2-tailed); highly negative correlation (significant) between Zn and Cu/Zn (r=0.65), p value at the 0.01 level (2-tailed); while weak negative or positive correlation (insignificant) between other parameter levels in group one. Highly positive correlation (significant) between Cu and Cu/Zn (r=0.64), p value at the 0.01 level (2-tailed); highly negative correlation (significant) between Zn and Cu/Zn (r=0.57), p value at the 0.01 level (2-tailed); positive correlation (significant) between Cu and TAC, total protein, Se (r=0.34, 0.33, 0.37) respectively, p value at the 0.05 level (2-tailed); while weak negative or positive correlation (insignificant) between other parameter levels in group two. Highly positive correlation (significant) between Cu and Cu/Zn (r=0.73), p value at the 0.01 level (2-tailed); highly negative correlation (significant) between Zn and Cu/Zn (r=0.58), p value at the 0.01 level (2-tailed); while weak negative or positive correlation (insignificant) between other parameter levels in group three. These results indicated that oral Montelukast had some undesirable effect on copper status than inhaled salbutamol.

روابط وملفات

التعريف والنوع

رقم الوثيقة

02329d03-c409-4f36-89ec-79bfce78429a

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل ماجيستير

نوع الملف

pdf text

أسماء الملفات

999683_1.pdf

بيانات النشر

ترجمة العنوان

تأثير الأدوية المضادة للربو على إنزيم النحاس – خارصين السوبر اوكسايد ديسميوتيز وبعض المتغيرات في مدينة الحلة

ألقاب المؤلفين

[{"name_ar":"رزاق شعلان خورشيد ألخالدي","title_ar":"اعداد","title_en":"Preparation"},{"name_ar":"عباس نور الشريفي","title_ar":"اشراف","title_en":"Supervision"},{"name_ar":"مها فاضل سميسم","title_ar":"اشراف","title_en":"Supervision"}]

اللغة

English

المصدر والدورية

اسم المصدر

Effect of Antiasthmatic Drugs on Cu-Zn Superoxide Dismutase and other Parameters in Hilla City

المحتوى والصفحات

عدد الصفحات

0

كلمات الباحثين

Asthma, Antioxidants, Salbutamol, Montelukast, Antioxidant enzymes, Copper, Zinc, Selenium, Superoxide dismutase

إشراف وإعداد

الإشراف

عباس نور الشريفي, مها فاضل سميسم

الإعداد

رزاق شعلان خورشيد ألخالدي

الاقتباسات الببليوغرافية