رسائل ماجيستير
English
2005
Isolation and Characterization of Bacteria from Patients with Conjunctivitis in Hilla Province
قاسم نجم الربيعي, محمد صبري, نيران كاظم فرهود الربيعي
كلية الطب-جامعة بابل · العراق
In this study, 120 conjunctival swabs were subjected to isolate and identify the bacterial strains associated with conjunctivitis. These swabs were obtained from patients suffering from conjunctival inflammation of age groups ranging from 4 days to 74 years of both sexes who were attended to the Hilla Teaching Hospital / Ophthalmic Unit during the interval from November 2003 to May 2004 in Hilla province. Bacterial infections were detected in 82 (68.3%) samples, and no growth resulted in 38 (31.7%) samples. It was indicated that Haemophilus influenzae was the first colonizer of the conjunctival sac (49 isolates) among the children and adults, followed by Staphylococcus aureus (19 isolates), Streptococcus pneumoniae (7 isolates), and a few number (4 isolates) of Neisseria gonorrhoeae in the first week of life, an act which confirmed the predominance of Gram-negative bacteria early in life. Also, 3 isolates of Klebsiella pneumoniae were isolated from children below one year old and from adults.
Some virulence factors of these bacterial isolates were investigated, and the results showed that:
All isolates of Haemophilus influenzae and Neisseria gonorrhoeae could not possess a capsule, while all Klebsiella pneumoniae isolates possessed a capsule, and it was found out that 42.1% of Staphylococcus aureus isolates possessed a capsule, whereas only 28.6% of Streptococcus pneumoniae possessed a capsule.
The results also showed that all isolates of Haemophilus influenzae, Neisseria gonorrhoeae, and Klebsiella pneumoniae could not produce hemolysin, while all Streptococcus pneumoniae isolates were able to produce hemolysin, and only 68.4% of Staphylococcus aureus isolates were able to produce hemolysin.
All isolates of Haemophilus influenzae, Streptococcus pneumoniae, and Neisseria gonorrhoeae were not able to produce siderophores, but all Klebsiella pneumoniae isolates were able to produce siderophores, and only 26.3% of Staphylococcus aureus isolates were able to produce siderophores.
The results also showed that all isolates of Haemophilus influenzae, Streptococcus pneumoniae, and Neisseria gonorrhoeae were able to produce extracellular proteases, but all Klebsiella pneumoniae isolates were not able to produce extracellular proteases, whereas 42.1% of Staphylococcus aureus isolates were able to produce extracellular proteases.
All isolates of Haemophilus influenzae, Streptococcus pneumoniae, Neisseria gonorrhoeae, and Klebsiella pneumoniae had adherence factors, whereas all Staphylococcus aureus isolates did not have adherence factors.
Also, in vitro effect of some antibiotics on these bacterial isolates was studied, and the results showed that Ciprofloxacin and Chloramphenicol were the most sensitive among the other drugs; hence, they should be the first choice for the treatment of bacterial conjunctivitis.