رسائل ماجيستير
English
2014
دراسة تحليلية للتفاعل بين كرتين قابلتين للاختراق
ابراهيم عبدالكريم ابراهيم صلاح, خولة قمحية
جامعة القدس · فلسطين
viiAbstractThethesis's goal is to improve our understanding of the interaction betweenPAMAMdendrimer, dendrimer/DNA complex, dendrimer/DNA aggregate(many dendrimer/DNA complexes) and a soft particlelike a cell or aprotein in salt solution. The electrostatic interactionsbetween soft particles,such as dendrimersof different generations,or proteins, orcells,have been studied and calculated by implementing a new developed theoretical model derived by Ohshima. It describesthe electrostatic interaction between two soft charged spheres during three stages:before penetration, through penetration, and after penetration. It was found that the maximum interactionforcebetween two interpenetrating soft spheres occursinthe second third of regionofthefullpenetration(diameter of the smallest sphere). The electrostatic interaction decreases by increasing the concentration of the salt solution, but it is heavily dependent on the size of the particles whenvaryingthe concentration. Interactionsbetweendendrimer/DNA complexes, aggregates,andothersoftparticles are affected bythe degree of theDNA wrapping aroundthedenrimer, and this wrapping length increases by the increasing of dendrimer generation. Thisanalytical study expandsour understanding of the interaction between various soft particles in gene therapy.
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