Comparative Degradation of Emerging Pollutants Using Chemical and Enzymatic Approaches

الموضوعات

علوم بحتة وطبيعية

الملخص

Organic pollutants, especially those found in water bodies; pose a direct threat to various aquatic organisms as well as humans. A variety of different remediation approaches, including chemical and biological methods have been developed for the degradation of these organic pollutants. However, comparative mechanistic studies of pollutant degradation by these different systems are almost non-existent. In this study, the degradation of two emerging pollutants an antibiotic pollutant, Sulfamethoxazole (SMX) and a model thiazole pollutant, Thioflavin T (ThT), was carried out using advanced oxidation process (AOP), using UV+H2O2 or a peroxidase enzyme system. Optimization conditions for Sulfamethoxazole degradation by peroxidase enzyme showed an absolute requirement for a redox mediator (Hydroxybenzotriazole) as well as low pH for pollutant degradation. The other conditions for the efficient degradation were as follows: the concentration of hydrogen peroxide, peroxidase enzyme and SMX needed were 56 μM, 78 nM and 5 ppm respectively. The degradation of both pollutants was followed using UV-Vis spectrophotometry and liquid chromatography-mass spectroscopy (LC-MS), and the products formed were identified using tandem liquid chromatography-mass spectrometry-mass spectrometry (LC-MS-MS). The results showed that the two remediation approaches UV+H2O2 and peroxidases produced different sets of intermediates suggesting that different degradation schemes were operating in the two systems (AOP vs. Peroxidase enzyme system). Phytotoxicity studies carried out using Lactuca sativa (lettuce) showed different levels of detoxification of the pollutants by the two different remediation approaches. This is the first time that a detailed comparative study showing in detail the intermediates generated in chemical and biological remediation methods has been presented. Furthermore, the results show that different remediation systems have very different degradation schemes and result in products having different toxicities.

روابط وملفات

التعريف والنوع

رقم الوثيقة

fec9cb2d-9ed9-41f2-ac5e-6e82e2e33c77

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل ماجيستير

نوع الملف

pdf text

أسماء الملفات

7394_1.pdf

بيانات النشر

ترجمة العنوان

مقارنة تحلل الملوثات العضوية الناشئة باستخدام العامل الكيميائي والعامل الانزيمي

ألقاب المؤلفين

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اللغة

English

المصدر والدورية

اسم المصدر

Comparative Degradation of Emerging Pollutants Using Chemical and Enzymatic Approaches

المحتوى والصفحات

عدد الصفحات

0

إشراف وإعداد

الإشراف

سيد سلمان أشرف

الإعداد

خديجة أبو بكر عبدالرحمن المقضي

الاقتباسات الببليوغرافية