Degradation of various Emerging Pollutants by Recombinant Bacterial DyP Peroxidases

الموضوعات

علوم تطبيقية وتكنولوجية

الملخص

In recent years, concerns are being raised about the potential harmful effects of emerging pollutants (EPs) on human and aquatic lives. Extensive research is being conducted on developing treatment approaches to target this new class of toxic pollutants. Studies focused on biological (enzyme-based) methods have shown a potential as greener and possibly more economical alternatives to other treatment approaches, such as chemical methods. Peroxidase enzymes are being exploited for water remediation and have proven to be effective in degrading a variety of EPs present in water. However, their high cost still represents a major bottleneck towards their extensive use and exploitation in water remediation. Recent advances in recombinant DNA technology can be used to produce a large number of active enzymes at low costs. The current study focused on the use of recombinantly produced novel members of bacterial peroxidases, named dye decolorizing peroxidases, to study their effectiveness in degrading a number of diverse EPs. In this context, a sensitive bioanalytical MRM-based assay was developed to detect a mixture of 31 EPs and to examine their potential degradation by a panel of bacterial rDyPs. The results showed that not all rDyPs behaved similarly in their abilities to degrade EPs. Some rDyPs showed a promising potential to degrade some EPs while others were ineffective. Additionally, the role of redox mediators for effective EP degradation by rDyPs was also examined. Our studies showed for the first time that recombinant bacterial DyPs can be used to degrade caffeic acid, 2-mercaptobenzothiazole, acrylamide, gemfibrozil, sulfamethoxazole, venlafaxine, biochanin A, prometryn and 3-Methyl-2benzothiazolinone hydrazone, MCPA, penicillin GK and phenytoin.

روابط وملفات

التعريف والنوع

رقم الوثيقة

fe691e42-6dae-45a5-a221-3420f7012e04

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل ماجيستير

نوع الملف

pdf text

أسماء الملفات

2283011_1.pdf

بيانات النشر

ألقاب المؤلفين

[{"name_ar":"Ahmed Yousef","title_ar":"اشراف","title_en":"Supervision"},{"name_ar":"Ahsan Qurashi","title_ar":"اشراف","title_en":"Supervision"},{"name_ar":"Aya Alsadik","title_ar":"اعداد","title_en":"Preparation"},{"name_ar":"Syed Salman Ashraf","title_ar":"اشراف","title_en":"Supervision"}]

اللغة

English

المصدر والدورية

اسم المصدر

Degradation of various Emerging Pollutants by Recombinant Bacterial DyP Peroxidases

المحتوى والصفحات

عدد الصفحات

0

كلمات الباحثين

emerging pollutants, wastewater, DyP, bacterialperoxidase,LCMSMS

إشراف وإعداد

الإشراف

Ahmed Yousef, Ahsan Qurashi, Syed Salman Ashraf

الإعداد

Aya Alsadik

الاقتباسات الببليوغرافية