Hepatic Cytochromes P450 Enzyme Activities of UAE Coastal Rabbit Safi Fish Siganus canaliculatus

الموضوعات

علوم بيئية

الملخص

The cytochrome P450 dependent metabolism of benzo(a)pyrene and other xenobiotics has been investigated in liver microsomes prepared from a local marine Safi fish Siganus canaliculatus caught from the Arabian Gulf of United Arab Emirates. The Safi fish was found to have a well developed liver microsomal monooxygenase system consisting of cytochrome P450, cytochrome b5 and NADPH-cytochrome c reductase. The fish microsomal enzyme system was able to metabolise benzo(a)pyrene, 7-ethoxycoumarin and 7- ethoxyresorufin. Male fish were found to exhibit a higher monooxygenase activity than female fish. Treatment of fish with β-naphthoflavone was found to induce (2-4 fold) the activities of aryl hydrocarbon hydroxylase, ethoxycoumarin O-deethylase and ethoxyresorufin O-deethylase. In vitro addition of α-naphthoflavone, a specific inhibitor of β-naphthoflavone inducible cytochrome P450s activities was found to inhibit the enzyme activities. These findings suggest the presence of various forms of cytochrome P450 in Safi fish liver. Western immunoblot analysis of liver microsomes from β-naphthoflavone treated fish using an antibody to rat liver cytochrome P450 1A1 (CYP1A1) suggested the presence of an inducible cytochrome P450 enzyme which was homologous to the mammalian CYP1A1 and was comparable with that of rat liver enzyme. Our results suggest that liver microsomes from the Safi fish have multiple forms of cytochrome P450 with a specific β-naphtho-flavone inducible CYP1A 1 homologous protein that can metabolise a variety of carcinogenic and non-carcinogenic substrates. Our results also indicate that like rats and mice and some other marine fish, Safi fish could also be used as a sensitive tool for the monitoring of the Arabian Gulf region pollution, especially with polycyclic hydrocarbons of the coastal water.

روابط وملفات

التعريف والنوع

رقم الوثيقة

fe4564fa-f587-4397-9126-a8473c88c114

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل ماجيستير

نوع الملف

pdf text

أسماء الملفات

7865_1.pdf

بيانات النشر

ألقاب المؤلفين

[{"name_ar":" Haider Raza","title_ar":"اشراف","title_en":"Supervision"},{"name_ar":"Amna Mana Al-Otaiba","title_ar":"اعداد","title_en":"Preparation"}]

اللغة

English

المصدر والدورية

اسم المصدر

Hepatic Cytochromes P450 Enzyme Activities of UAE Coastal Rabbit Safi Fish Siganus canaliculatus

المحتوى والصفحات

عدد الصفحات

0

إشراف وإعداد

الإشراف

Haider Raza

الإعداد

Amna Mana Al-Otaiba

الاقتباسات الببليوغرافية