Magnetic Nanowires as Materials for Cancer Cell Destruction

الموضوعات

علوم بحتة وطبيعية

الملخص

Current cancer therapies are highly cytotoxic and their delivery to exclusively the affected siteis poorly controlled, resulting in unavoidable and often severe side effects. In an effort to overcomesuch issues, magnetic nanoparticles have been recently gaining relevance in the areas of biomedicalapplications and therapeutics, opening pathways to alternative methods. This led to the concept ofmagnetic particle hyperthermia in which magnetic nano beads are heated by a high power magneticfield. The increase in temperature kills the cancer cells, which are more susceptible to heat incomparison to healthy cells.In this dissertation, the possibility to kill cancer cells with magnetic nanowires is evaluated.The idea is to exploit a magnetomechanical effect, where nanowires cause cancer cell death throughvibrating in a low power magnetic field. Specifically, the magnetic nanowires effects to cells in cultureand their ability to induce cancer cell death, when combined with an alternating magnetic field, wasinvestigated.Nickel and iron nanowires of 35 nm diameter and 1 to 5 μm long were synthesized by electrodepositioninto nanoporous alumina templates, which were prepared using a two-step anodizationprocess on highly pure aluminum substrates. For the cytotoxicity studies, the nanowires were addedto cancer cells in culture, varying the incubation time and the concentration. The cell-nanowireinteraction was thoroughly studied at the cellular level (mitochondrial metabolic activity, cell membraneintegrity and, apoptosis/necrosis assay), and optical level (transmission electron and confocalmicroscopy). Furthermore, to investigate their therapeutic potential, an alternating magnetic fieldwas applied varying its intensity and frequency. After the magnetic field application, cells healthwas measured at the mitochondrial activity level.Cytotoxicity results shed light onto the cellular tolerance to the nanowires, which helped inestablishing the appropriate nanowire concentrations to use the nanowires + alternating magneticfield combination as a cancer treatment. Different levels of cancer cell death were achieved bychanging the incubation time of the nanowires with the cells and the alternating magnetic fieldparameters. Cell viability was significantly affected in terms of mitochondrial activity and cellmembrane integrity after applying the treatment (nanowires + alternating magnetic field) using alow-frequency alternating magnetic.Theoretical calculations considering the magnetic and viscous torques showed that the nanowiresvibrate as a consequence of the applied magnetic field. This, alongside the fact that no temperatureincrease was measured during the treatment, makes the magnetomechanical effect the most probableaction mechanism in the applied treatment that is inducing cell death.Inducing cancer cell death via magnetomechanical action using magnetic nanowires resulted inkilling up to 60% of cancer cells with only 10 minutes of treatment. The required magnetic field fortreatment is in a low power regime, which is safe, does not cause any discomfort to the patients,and can be generated with compact and cheap instruments.

روابط وملفات

التعريف والنوع

رقم الوثيقة

fda0558c-6532-47c7-b5ee-25c7ee351283

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل دكتوراة

نوع الملف

pdf text

أسماء الملفات

1700022_1.pdf

بيانات النشر

ألقاب المؤلفين

[{"name_ar":"تيموسي رافاياس","title_ar":"اشراف","title_en":"Supervision"},{"name_ar":"ماريا كونتيراس","title_ar":"اعداد","title_en":"Preparation"}]

اللغة

English

المصدر والدورية

اسم المصدر

Magnetic Nanowires as Materials for Cancer Cell Destruction

المحتوى والصفحات

عدد الصفحات

0

كلمات الباحثين

magnetic field, Magnetic nanowires

إشراف وإعداد

الإشراف

تيموسي رافاياس

الإعداد

ماريا كونتيراس

الاقتباسات الببليوغرافية