Maternal Iimmunity to Newcastle Disease in Egg Yolk of layers

الموضوعات

طب

الملخص

This study was carried out on ninety six egg samples collected from laying hensfrom commercial chicken farm at Alhalfaia (Khartoum North). The hens werevaccinated against Newcastle disease (ND) with the Newcastle disease virus (NDV)vaccine (Komorov strain) produced in the Central Veterinary Research Laboratoriescenter (Khartoum, Sudan).This experimentattempt to detect the presence ofimmunoglobulin Y (IgY) in the egg yolk as a result of transfer of maternalantibodies from immunized hen’s sera to the egg yolk and to study the specificity ofthese antibodies to NDV.The immunoglobulin Y(IgY) was extracted from egg yolkby dextran sulphate method. The method of extraction consists of three steps:Precipitation of lipids by dextran sulphate; precipitation of proteins by sodiumsulphate; and dialysis of precipitateagainst tris- buffered saline (TBS).The presence of IgY in egg yolk, antibody titers and specificity to Newcastledisease virus (NDV) were determined by standardized indirect ELISA (In- houseELISA). All Ninety six samples were found positive (100%). The positive resultswere varied in titers between log213.643 (the maximum) and log28.643 (theminimum). As comparative study, detection of maternal specific NDV IgY wasdone using NDV antibody test kit. All samples tested were found positive andantibody titers were varied between log214.171 (the maximum) and log210.838 (theminimum). It was found that titers obtained from NDV antibody testkit were higherthan those obtained from the standardized indirect ELISA. From this study, it wasfound that maternally derived IgY antibodies present in the egg yolk specific toNDV were present in high titers that confer protection during early weeks of life ofnewly hatched chicks. Extraction of maternally derived specific NDV antibodiesfrom yolk will facilitate accurate monitoring of ND vaccination programmes.

روابط وملفات

التعريف والنوع

رقم الوثيقة

fd680a65-1c8b-4110-805f-9228a3d4aec5

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل ماجيستير

نوع الملف

pdf text

أسماء الملفات

1637978_1.pdf

بيانات النشر

ألقاب المؤلفين

[{"name_ar":" Awad Alkarim Abdelghaffar Ibrahim","title_ar":"اشراف","title_en":"Supervision"},{"name_ar":"Amal Abu Baker Mohamed Bashir ","title_ar":"اعداد","title_en":"Preparation"}]

اللغة

English

المصدر والدورية

اسم المصدر

Maternal Iimmunity to Newcastle Disease in Egg Yolk of layers

المحتوى والصفحات

عدد الصفحات

0

إشراف وإعداد

الإشراف

Awad Alkarim Abdelghaffar Ibrahim

الإعداد

Amal Abu Baker Mohamed Bashir

الاقتباسات الببليوغرافية