Experimental evaluation of P.berghei sporozoites in light of selected innate immune components

الموضوعات

علوم بحتة وطبيعية

الملخص

Known as polymorphonuclear leukocytes (PMNs), neutrophils have been shown to play a regulatory role in healing injuries and resolving infections. Although the immune response (IR) of neutrophils in bacterial and fungal infections is well understood, their impact during sporozoite infection is much less studied. This project investigates the role of neutrophils in recognizing Plasmodium berghei sporozoites in an effort to understand the mechanism of sporozoites’ IR evasion and spread of malaria infection in the blood circulation. Plasmodium berghei GFP-expressing sporozoites were purified from infected mosquitoes by manual dissection of salivary glands and exposed to antibiotics for decontamination. Processed and post-thaw cryopreserved sporozoites were assessed for viability using LSM and quantified using hematocytometer. Viable sporozoites were then incubated with human host blood, mimicking in vivo infection, and evaluated using the neutrophil function test (NFT) and spectral resolution. NFT results showed an activation rate of neutrophils of about 70% and incomplete loss of GFP signal upon incubation with sera complement proteins. This output delineates a partial early IR induced by neutrophils, leaving space for ambiguous queries related to the factors hindering a complete protective efficacy.

روابط وملفات

التعريف والنوع

رقم الوثيقة

fd48d46f-ca15-46f2-8f75-a714c9a4a3f9

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل ماجيستير

نوع الملف

pdf text

أسماء الملفات

2294369_1.pdf

بيانات النشر

ألقاب المؤلفين

[{"name_ar":" Zeina Antoine Rhaim","title_ar":"اعداد","title_en":"Preparation"},{"name_ar":"Ghanem Esther","title_ar":"اشراف","title_en":"Supervision"}]

اللغة

English

المصدر والدورية

اسم المصدر

Experimental evaluation of P.berghei sporozoites in light of selected innate immune components

المحتوى والصفحات

عدد الصفحات

0

كلمات الباحثين

Neutrophils Natural immunity Complement (Immunology) Plasmodium

إشراف وإعداد

الإشراف

Ghanem Esther

الإعداد

Zeina Antoine Rhaim

الاقتباسات الببليوغرافية