Assessment of Molecular Diagnosis Methods for Abdominal Tuberculosis and Tuberculous Lymphadenitis among Patients in Gezira State, Sudan

الموضوعات

طب

الملخص

Extrapulmonary tuberculosis is considered an important clinical problem. The aim of this study was assess two molecular methods kit (CinnaGen Inc Iran) and IS6110 gene and two different samples (blood and fluid) for the diagnosis of abdominal tuberculosis and tuberculous lymphadenitis. The study subjects were recruited from Wad Medani teaching hospital during 2009-2013. Seventy five ascitic fluid and blood samples were collected from each patients suspected to have abdominal tuberculosis and twenty five lymph aspirate and blood were collected from each suspected tuberculous lymphadenitis. DNA was extracted using DNPTM kit (CinnaGen Inc) and polymerase chain reaction (PCR) was done using a kit (CinnaGen Inc) and IS6110 gene for both samples. In abdominal tuberculosis, the comparison between ascitic fluid and blood samples when kit were used, 10/75 (13%) and 3/75 (4%) were positive respectively, when IS6110 gene used, 20/75 (27%) and 9/75 (12%) were positive respectively, there was a significant difference in both results, P-value 0.05, The correlation between the clinical presentation of the study subjects and PCR using IS6110 gene, showed that 12/75 (16%) were clinically highly suggestive while 20/75 (27%) were positive a significant difference was found, P- Value 0.05 in both cases. The correlation between the clinical presentation of the study subjects and PCR using IS6110 gene, showed that 13/25 (52%) of the tuberculous lymphadenitis cases were clinically highly suggestive, while 10/25 (40%) were positive with a significant difference P- Value < 0.05. This study conclude that the best sample for diagnosis of abdominal TB and Lymphadenitis is ascitic fluid and LN aspirate and the best diagnostic method to use is IS6110 gene. Clinical presentation is important to identify extrapulmonary tuberculosis cases. In this study, ascitic fluid and lymph aspirate samples is recommended to be used in molecular diagnostic test. IS6110 insertion sequence gene is the best method to be used. Clinician should put more emphasis in the on clinical criteria for diagnosis extrapulmonary tuberculosis.

روابط وملفات

التعريف والنوع

رقم الوثيقة

fcf90e1f-2db9-49c8-9374-8795fb438c1a

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل ماجيستير

نوع الملف

pdf text

أسماء الملفات

72463_1.pdf

بيانات النشر

ترجمة العنوان

تقييم طرق التشخيص الجزيئي المختلفة في في تشخيص السل البطني وسل العقد اللمفية في مرضى ولاية الجزيرة –السودان

ألقاب المؤلفين

[{"name_ar":"الجليلي محمد الجليلي الأمين","title_ar":"اشراف","title_en":"Supervision"},{"name_ar":"عثمان خلف الله سعيد طه","title_ar":"اشراف","title_en":"Supervision"},{"name_ar":"مؤيد عبدالسلام الحاج الجاك","title_ar":"اعداد","title_en":"Preparation"},{"name_ar":"نجلاء حاسم السيد محمد أحمد","title_ar":"اشراف","title_en":"Supervision"}]

اللغة

English

المصدر والدورية

اسم المصدر

Assessment of Molecular Diagnosis Methods for Abdominal Tuberculosis and Tuberculous Lymphadenitis among Patients in Gezira State, Sudan

المحتوى والصفحات

عدد الصفحات

0

إشراف وإعداد

الإشراف

الجليلي محمد الجليلي الأمين, عثمان خلف الله سعيد طه, نجلاء حاسم السيد محمد أحمد

الإعداد

مؤيد عبدالسلام الحاج الجاك

الاقتباسات الببليوغرافية