Whole Genome Molecular Characterization of Methicillin Resistant Staphylococcus aureus Isolated From Clinical Settings in Lebanon

الموضوعات

علوم بحتة وطبيعية

الملخص

The high prevalence of multi-drug resistant Staphylococcus aureus, particularly the Methicillin-Resistant S. aureus (MRSA), has become a worldwide health concern. In this study, we used whole-genome sequencing, multi-locus Sequence Typing (MLST), S. aureus protein A (spa), and Staphylococcal Chromosomal Cassette (SCCmec) typing of MRSA and pan-genome analysis to study the molecular characteristics, resistance patterns, and relatedness of the isolates. In silico typing was used to determine the antimicrobial resistance and virulence determinants, rep families, and prophages. All the studied isolates carried the blaZ gene, and most were multi-drug resistant. Two MRSA isolates recovered from hospitalized patients were additionally resistant to vancomycin. Seven distinct MLST allelic profiles were identified, and the most common STs and spa types, respectively, were ST789 and ST1and t091 and t127. SCCmec typing of MRSA revealed the prevalence of type V(5C2). MRSA ST789-t091-V was the predominant type, but we also detected SCCmec cassette harboring ccrA1B1 and mecC2, classified as SCCmec type IX(1C2).

روابط وملفات

التعريف والنوع

رقم الوثيقة

fc850242-1eb4-4d5f-8fc4-7be6836133a3

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل دكتوراة

نوع الملف

pdf text

أسماء الملفات

2212697_1.pdf

بيانات النشر

ألقاب المؤلفين

[{"name_ar":" Tia Almachtoub","title_ar":"اعداد","title_en":"Preparation"},{"name_ar":"Sima Tokajian ","title_ar":"اشراف","title_en":"Supervision"}]

اللغة

English

المصدر والدورية

اسم المصدر

Whole Genome Molecular Characterization of Methicillin Resistant Staphylococcus aureus Isolated From Clinical Settings in Lebanon

المحتوى والصفحات

عدد الصفحات

0

كلمات الباحثين

SCCmec

إشراف وإعداد

الإشراف

Sima Tokajian

الإعداد

Tia Almachtoub

الاقتباسات الببليوغرافية