Genetic Characterization of Ana plasma and Ehrlichia in Ixodid Ticks and Animals from Palestine: SpatialDistribution

الموضوعات

علوم بحتة وطبيعية

الملخص

Background: Tick-borne anaplasmosis and ehrlichiosis are rickettsial diseases caused by various species in the genera Ehrlichiaand Anaplasma causing different emerging diseases worldwide with significant negative impacts on both human and animals. This study aimed at detecting and genetically characterizing of Ehrlichiaand Anaplasma species in ixodidticks and their animal hosts from Palestine. Methodology/ Principal Findings:A total of 723 ixodidticks belonging to seven species (Rhipicephalus sanguineus, Rhipicephalus turanicus, Rhipicephalus bursa, Hyalomma dromedarii, Hyalomma impeltatum, Haemaphysalis parva, Haemaphysalis adleri) were collected from dogs, sheep, goats and camels. In addition, 189 blood samples were collected from different animal hosts including; dogs, sheep, camels, horses and a goat residing in nine districts in Palestine. DNA was extracted from all samples (ticks and blood) and screened for the presence of Anaplasmaand Ehrlichiatargeting a 345-partial sequence of the 16s rRNAgene followed by sequence analysis. Anaplasma DNA was detected in 6.5% (47/723) of the tested ticks. Among them,13 were Anaplasma platysand 34 were Anaplasma spp. Using short msp4-PCR, 24 samples were further identified as Anaplasma ovis.Four ticks were infected with Ehrlichia (4/723; 0.6%), three of them wereEhrlichia canis and one was Ehrlichia spp.Among canine blood samples (n=135), 11.1% (15/135) were positive. Of which, 13 (9.6%) samples were infected with A. platysand two samples (1.5%) were infected with Ehrlichia canis. Out of 47 sheep blood samples, 19 (40.4%) samples were positive; 6 of them were identified as Anaplasma spp. and 13 samples were found to be infected with A. ovisusing our newly designed primers targeting a partial sequence of msp4 gene. Implementation of purely-spatial analysis by saTScan for the pooled cases ofAnaplasmarevealed two statistically significant clusters in two districts in Palestine; Tubas and Majdal-Prepared by Supervisor Co-supervisor : Taher Mohammad Taher Zaid : Dr. Suheir Ereqat : Dr. Abedelmajeed Nasereddin iv Bani-Fadil village on the western hills of the Jordan Valley. Most cases (83%) were in rural areas where life cycle components abundantly interact.Conclusions:This study revealed the presence of Anaplasmaand Ehrlichiain Palestine. Public health workers should be aware of emerging tick-borne diseases in Palestine, particularly infections due to A. ovis.

روابط وملفات

التعريف والنوع

رقم الوثيقة

fbb89445-b390-447f-9097-71ff01b0d0c6

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل ماجيستير

نوع الملف

pdf text

أسماء الملفات

1696910_1.pdf

بيانات النشر

ترجمة العنوان

التوصيف الوراثي للبكتيريا من الجنسين والحيوانات العائلة لها في فلسطين: التوزيع المكاني

ألقاب المؤلفين

[{"name_ar":"سهير عريقات","title_ar":"اشراف","title_en":"Supervision"},{"name_ar":"طاهر محمد طاهر زيد","title_ar":"اعداد","title_en":"Preparation"}]

اللغة

Arabic

المصدر والدورية

اسم المصدر

Genetic Characterization of Ana plasma and Ehrlichia in Ixodid Ticks and Animals from Palestine: SpatialDistribution

المحتوى والصفحات

عدد الصفحات

0

إشراف وإعداد

الإشراف

سهير عريقات

الإعداد

طاهر محمد طاهر زيد

الاقتباسات الببليوغرافية