Molecular and Morphological Comparative Study Of Echinococcus spp. In Intermediate Hosts In Al-Nasiriyah City, South of Iraq

الموضوعات

علوم بحتة وطبيعية

الملخص

The current study aimed to find out the extent of the epidemiology of hydatid cysts disease caused by the larval stage of the parasite Echinococcus granulosus that affects humans and different animals and measure the epidemiology among animals slaughtered in the slaughterhouses of the Al-Nasiriyah city, Thi-Qar province, markets butchers and butchers working in markets selling livestock, in addition to human infected cases that taken from civil and government hospitals in Al-Nasiriyah city, Thi-Qar province, and the epidemiology of the parasite in the final host (stray dogs feces) to find the eggs of the parasite, and make a morphological and molecular comparative study to the protoscolices from the hydatid cyst from intermediate hosts. In this study, (910) cases were examined by direct manual examination after the slaughter was completed by the butchers, and found 42 cases infected with hydatid cysts distributed as follows (11 cows, 3 Buffalos, 6 camels, 16 sheep and 6 human cases) and different infection rates of E. granulosus in cow, buffalo, camel, sheep, goats and human were found to be 3.5% (11/312), 4.5% (3/66), 16.7% (6/36), 4.6% (16/346), 0.0% (0/144) and 100% (6/6), respectively. The different infection rates where the highest infection in camels was 16.7% and the lowest infection was in cows 3.5%. There is high significant difference in the infection among different animals at probability level ≤ 0.01. There are 12 samples are proved to contain eggs of the parasite from 89 samples was collected from stray dogs feces that live and roam near the butchers working in the local market for selling sheep and goats in the south of Al-Nasiriyah city, Thi-Qar province. Measurements of the small and large hooks of the protoscolices that isolated from different organs in the various infected intermediate hosts showed significant differences in the length and width of the hooks in addition to the length of the handle. After molecular diagnosis was completed using polymerase chain reaction technology (PCR) with the Cox1gene in the gel electrophoresis technique by using the primers JB3 and JB4.5, the current study found that all infected cases of intermediate hosts belong to strains of the genus Echinococcus spp. The highest purity of the DNA used in the polymerase chain reaction technique was obtained by the using protoscolices as a source of DNA, where the extraction results showed that the use of protoscolices as a source of DNA is better than the use of germinal layer tissues, because of the easy analysis of protoscolices in compared to germinal layer tissues. The analysis of the results of the genetic sequence alignment of the Cox1 gene showed that all of the genotypes G1, G3 and G6 are responsible for the infection of hydatid cyst in humans and livestock such as sheep, buffalo, cow and camel, as it was proved that these types are the most infected cases with cystic echinococcosis. As the current study is a preliminary study to detect the strains of Echinococcus spp. that infect intermediate hosts in Al-Nasiriyah city, the camel strain G6 was recorded for the first time in Al-Nasiriyah city in human liver by based on the analysis of the Cox1 gene sequence. The results of the phylogenetic tree analysis showed that there is a genetic affinity of 86-100% between the local isolates that were isolated from different hosts and from different organs of the same animal.

روابط وملفات

التعريف والنوع

رقم الوثيقة

fab346c6-f95b-4234-93bb-35a97ad49541

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل ماجيستير

نوع الملف

pdf text

أسماء الملفات

2153797_1.pdf

بيانات النشر

ترجمة العنوان

دراسة جزيئية ومظهرية مقارنة لطفيلي المشوكة Echinococcus spp في المضائف الوسطية في مدينة الناصرية- جنوب العراق

ألقاب المؤلفين

[{"name_ar":"حبيب وسيل كاظم شبر","title_ar":"اشراف","title_en":"Supervision"},{"name_ar":"حسين صالح مهدي اليعقوبي","title_ar":"اعداد","title_en":"Preparation"}]

اللغة

English

المصدر والدورية

اسم المصدر

Molecular and Morphological Comparative Study Of Echinococcus spp. In Intermediate Hosts In Al-Nasiriyah City, South of Iraq

المحتوى والصفحات

عدد الصفحات

0

إشراف وإعداد

الإشراف

حبيب وسيل كاظم شبر

الإعداد

حسين صالح مهدي اليعقوبي

الاقتباسات الببليوغرافية