رد الفعل المناعي تجاه الليشمانيا المثبطة بالميتوميسين علي مستوي العقد اللمفية النازحة لدي الفئران

الموضوعات

طب

روابط وملفات

التعريف والنوع

رقم الوثيقة

f5673b90-9080-45fe-98c2-6b0b44391036

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل ماجيستير

نوع الملف

pdf text

أسماء الملفات

1965927_1.pdf

بيانات النشر

ترجمة العنوان

The immune response against leishmania inhibited by mitomycin-c at the level of draining lymph nodes in mice.

ألقاب المؤلفين

[{"name_ar":"فاطمة محي الدين حسين","title_ar":"اعداد","title_en":"Preparation"},{"name_ar":"فيحاء أبو فخر","title_ar":"اشراف","title_en":"Supervision"},{"name_ar":"محمد معروف","title_ar":"اشراف","title_en":"Supervision"}]

اللغة

Arabic

المصدر والدورية

اسم المصدر

رد الفعل المناعي تجاه الليشمانيا المثبطة بالميتوميسين علي مستوي العقد اللمفية النازحة لدي الفئران

المحتوى والصفحات

عدد الصفحات

0

ترجمة الملخص

The Syria is considered to be endemic regions in cutaneous leishmaniasis. While there are not effective and safe drugs, the prevention is very important to stop dispersing the disease. Our study aimed to determine the capacity of mitomycin-c treated live leishmania promastigotes to produce the immunity and studying skin lesions volume and parasite load in the lesions and draining lymph nodes and immunophenotyping by flowcytometry and gene expression of mRNA for cytokines at the level of draining lymph nodes by Real-time PCR in BALB/C mice which were immunized with 104 mitomycin-c treated live leishmania promastigotes, or 104 heat- killed leishmania, or 104wild type leishmania promastigotes. 4 weeks after the immunizing, mice were injected with challenge, 106L. tropicametacyclic promastigotes, and were followed up at two weeks intervals for 8 weeks after challenge. 4 weeks after the immunizing our results revealed that mice immunized with wild type leishmania promastigotes showed lesions and parasite load in the site of injection and draining lymph nodes while those immunized with mitomycin-c treated live leishmania promastigotes or heat- killed leishmania do not show any skin lesions or parasite load in the site of injection and draining lymph nodes. our results revealed that increase numbers of helper T cells, cytotoxic T cells, B cells, natural killer cells, macrophages, and dentritic cells and increase gene expression of mRNA for IFN-γ and IL-12 in mice which were immunized with mitomycin-c treated live leishmania promastigotes comparable with mice which immunized with heat- killed leishmania or wild type leishmania promastigotes which indicated that mitomycin-c treated live leishmania promastigotes induce Th1 response. after challenge, lesions and parasite load in the site of injection showed in all groups and lesions size and parasite load began to increase at 8 weeks after challenge and were less in mice which were immunized with mitomycin-c treated live leishmania promastigotes. Beside of this, parasites showed in draining lymph nodes and parasite load began to 109decrease at 8 weeks after challenge and were less in mice which were immunized with mitomycin-c treated live leishmania promastigotes. Numbers of helper T cells increased and began to decrease after challenge which associated with increase of gene expression of mRNA for IL-4 in mice which were immunized with mitomycin-c treated live leishmania promastigotes and was less comparable with mice which immunized with heat- killed leishmania or wild type leishmania promastigotes. Numbers of cytotoxic T cells, B cells, natural killer cells, macrophages, and dentritic cells decreased and began to decrease after challenge which associated with decrease of gene expression of mRNA for IFN-γ and IL-12 in mice which were immunized with mitomycin-c treated live leishmania promastigotes and was less comparable with gene expression of mRNA for IL-4 which indicated that mitomycin-c treated live leishmania promastigotes induce Th2 response. Running title: The immune response against leishmania inhibited by mitomycin.

كلمات الباحثين

cutaneous leishmaniasis, gene expression and draining lymph nodes, mitomycin-c treated live leishmania promastigotes, parasite load, skin lesion, التعبير الجيني, حمل الطفيلي, داء الليشمانيات الجلدي, طفيليات الليشمانية المثبطة الانقسام بالميتوميسين الأذية الجلدية, والعقد المفية النازحة

إشراف وإعداد

الإشراف

فيحاء أبو فخر, محمد معروف

الإعداد

فاطمة محي الدين حسين

الاقتباسات الببليوغرافية