Detection, molecular typing and phylogenetic analysis of Leishmania isolated from cases of leishmaniasis among Syrian refugees in Lebanon

الموضوعات

علوم بحتة وطبيعية

الملخص

Leishmania is a parasitic protozoan with more than two-dozen species causing the disease leishmaniasis. It is transmitted to humans through the bite of an infected female phlebotomine sand-fly. In the past two years the incidence of leishmaniasis has been drastically increasing in Lebanon. This was in parallel with the deterioration of the security in Syria forcing thousands to flee seeking shelter at refugee camps in Lebanon. Water, sanitation and hygiene facilities in those collective shelters are far from adequate, increasing the risk and turning those camps into fertile grounds for the spread of diseases. Cutaneous leishmaniasis (CL) is now considered a public health problem, but its epidemiology has not been fully elucidated. A crucial point in containing the disease is first Leishmania species identification and then correlation with clinical parameters. In this study, two molecular typing methods for Leishmania speciation were used along with sequencing and phylogenetic analysis. The parasites identified were all Leishmania tropica as determined by both the ITS1-PCR RFLP and the nested ITS1-5.8S rDNA gene amplification. ITS1-5.8S rDNA gene based typing proved to be more sensitive in the detection of parasites (positive in 70% of the isolates) as opposed to the ITS1-PCR RFLP method that was successful in identifying Leishmania in only 47.4% of the isolates. Sequencing and phylogenetic analysis revealed high levels of heterogeneity among the sequenced isolates. To our knowledge, this is the first study using two different molecular methods for the detection and identification of L. tropica in Lebanon. The obtained results could be used for accurate detection and diagnosis of Leishmania in CL and for tracing possible changes in the population structure of L. tropica.

روابط وملفات

التعريف والنوع

رقم الوثيقة

f46cb146-1009-4c03-82e9-431d70ed76cc

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل ماجيستير

نوع الملف

pdf text

أسماء الملفات

2213122_1.pdf

بيانات النشر

ألقاب المؤلفين

[{"name_ar":" Sima Tokajian","title_ar":"اشراف","title_en":"Supervision"},{"name_ar":" Tamara Salloum","title_ar":"اعداد","title_en":"Preparation"}]

اللغة

English

المصدر والدورية

اسم المصدر

Detection, molecular typing and phylogenetic analysis of Leishmania isolated from cases of leishmaniasis among Syrian refugees in Lebanon

المحتوى والصفحات

عدد الصفحات

0

كلمات الباحثين

Syrian refugees in Lebanon

إشراف وإعداد

الإشراف

Sima Tokajian

الإعداد

Tamara Salloum

الاقتباسات الببليوغرافية