Induction, Elicitation and Determination of Total Anthocyanin Secondary Metabolites from In vitro Growing Cultures of Arbutus andrachne L.

الموضوعات

علوم بحتة وطبيعية

الملخص

Anthocyanin pigments are important secondary metabolites that produced in many plant species.They have wide range of uses in food and pharmaceutical industries as antioxidant and foodadditives. Medically, they prevent cardiovascular disease and reduce cholesterol levels as well asshow anticancer activity. This study aims at utilizing a rare medicinal tree, A. andrachne in theproduction of anthocyanin pigments via plant in vitro culture techniques. The effects of differentphysical and chemical factors on total anthocyanin content (TAC) in callus and cell suspensionculture of A. andrachne have been investigated. Anthocyanins have been detected in the callus ofA. andrachne with the presence of light. McCown Woody Plant medium (Mcc) supplementedwith 0.5 mg/l NAA, 2.00 mg/l TDZ and 1.0 g/l PVP resulted in red colored callus occurred after16 days of culture. The highest anthocyanin concentration was observed in MCc salt mixture,sucrose and a mix of starch and agar for gelling. Cell suspension growth and anthocyaninproduction was the highest in Gamborg’s B5 media that supplemented with 0.2mg/l Kineitin, 0.1mg/l NAA, 0.25 g/l casein hydrolysate and 0.3% w/v sucrose. The effect of osmotic stress byusing sorbitol enhanced the production of anthocyanin pigments at earlier stage than control(sucrose alone). Elicitation of anthocyanins with salicylic acid at 1.0 mg/l for 48 hours showedsignificant increase in TAC from 2.6 to 3.8 mg/l. This study outlined a protocol for large scaleanthocyanin production via plant in vitro culture technique.Key words: Anthocyanin, Arbutus andrachne L., callus culture, cell suspension culture,elicitation

روابط وملفات

التعريف والنوع

رقم الوثيقة

f4673181-6a47-4f06-846f-951016fcd53e

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل ماجيستير

نوع الملف

pdf text

أسماء الملفات

1819856_1.pdf

بيانات النشر

ألقاب المؤلفين

[{"name_ar":"Abla Ghassan Jaber","title_ar":"اعداد","title_en":"Preparation"},{"name_ar":"Rami Arafeh","title_ar":"اشراف","title_en":"Supervision"}]

اللغة

English

المصدر والدورية

اسم المصدر

Induction, Elicitation and Determination of Total Anthocyanin Secondary Metabolites from In vitro Growing Cultures of Arbutus andrachne L.

المحتوى والصفحات

عدد الصفحات

0

كلمات الباحثين

Science

إشراف وإعداد

الإشراف

Rami Arafeh

الإعداد

Abla Ghassan Jaber

الاقتباسات الببليوغرافية