Abnormal Antigens in Breast Cancer Tissues and Production of Monoclonal Antibodies Against one of these Antigens

الموضوعات

طب

الملخص

Back groundBreast cancer is associated with up regulation, down regulation of normalantigens or abnormal antigens. These antigens are very useful candidates astargets for the different breast cancer therapies and for vaccination trials.ObjectivesThis study was done to characterize abnormal antigens, extract one of themand to produce monoclonal antibodies against the extracted antigen.MethodsOne hundred and twenty Sudanese female patients were included in thisstudy after informed consent. The mean age was 47.2 years (16-80). Twotissue samples were obtained from each patient and they were confirmed asnormal and cancerous breast tissues microscopically. 2D PAGE was used toanalyze the protein content of samples. LC/MS and nr.fasta database searchwere used for separation and identification of the abnormal proteins.ResultsThree different patterns of 2D PAGE results were obtained, the firstpattern involved detection of four abnormal proteins in 26.7% of the patientcancerous tissues while they were undetected in the normal tissues of the samepatients. In the second 2D PAGE result pattern the cancerous and the normaltissues of 67.5% patients were identical and they did not contain the fourabnormal proteins while the third 2D PAGE pattern involved the presence oftwo abnormal antigens (from the four) in the cancerous tissues of 5.8% of thepatients and they were absent from the normal tissues of the same patients.The four abnormal proteins were identified as, human Thioredoxin (D60nXIImutant), X-ray crystal structure of human galectin-1, retrocopy oftropomyosin 3(rcTPM3) and beta-tropomyosin (isoform 2). The primary andthe secondary structures were obtained from the SWISSPROT and the PDBdatabases. Beta-tropomyosin spot was extracted and used as antigen formonoclonal antibody production. Monoclonal antibody against betatropomyosinwith a concentration of 0.35mg/ml and a G11 anti betatropomyosinhybridoma cell line were produced. The monoclonal antibodywas with single band and approximately 30 KDa molecular weight. However,some tests were done to determine the specificity of the produced monoclonalantibody.ConclusionsIn conclusion, breast cancer tissues are associated with abnormalantigens, but with low incidence rate; this strongly favors the individualizationof breast cancer diagnosis and treatment

روابط وملفات

التعريف والنوع

رقم الوثيقة

f3bd1e42-d068-4f38-a98c-7c4a76b1385c

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل دكتوراة

نوع الملف

pdf image

أسماء الملفات

f3bd1e42-d068-4f38-a98c-7c4a76b1385c_1.pdf

بيانات النشر

ألقاب المؤلفين

[{"name_ar":"Abdelrahim Osman Mohamed","title_ar":"اشراف","title_en":"Supervision"},{"name_ar":"Mohammed Elimam Ahamed Mohammed","title_ar":"اعداد","title_en":"Preparation"}]

اللغة

English

المصدر والدورية

اسم المصدر

Abnormal Antigens in Breast Cancer Tissues and Production of Monoclonal Antibodies Against one of these Antigens

المحتوى والصفحات

عدد الصفحات

0

إشراف وإعداد

الإشراف

Abdelrahim Osman Mohamed

الإعداد

Mohammed Elimam Ahamed Mohammed

الاقتباسات الببليوغرافية