Differential expression of the Bradykinin and Retinoic Acid Receptors in human colorectal cancer cell lines

الموضوعات

طب

الملخص

Chronic inflammation is a major characteristic of the development and progression of several tumors, in particular colorectal cancer. The connection between inflammation and tumorigenesis has been well supported from pharmacological, epidemiological, and genetic data. However, the molecular mechanism by which inflammation promotes cancer cell growth constitutes a broad area of ongoing investigation. Hence, multiple key genes and signaling pathways involved in oxidative stress and inflammation, such as the kallikrein-kinin system, may play a key role in tumorigenesis. In fact, bradykinin has been shown to modulate tumor progression. We have also shown that bradykinin activates the extracellular signaling regulated kinase (ERK) 1-2 pathway which may promotes cell survival or cell death in cancer cells. Retinoids are major regulators of epithelial cell proliferation, apoptosis, and differentiation and have therefore been used in the prevention and treatment of some cancers. Retinoids have also shown promise in preclinical colorectal cancer studies. Therefore, we were interested in investigating a potential crosstalk between the kallikrein-kinin system, and the retinoic acid signaling pathway using a well-characterized human in vitro colorectal cancer model. In the present study, we tested for the effects of bradykinin receptor 1 and 2 (B1R and B2R) agonists on the proliferation of human colorectal cancer cells, and on the expression and modulation of B1R, B2R, and retinoid receptors (RARα, RARγ, RXRα). We investigated as well the subcellular localization of RARγ, and B2R upon treatment with its high affinity ligand bradykinin, and some of the downstream effector signaling pathways. We showed by MTT assay that the growth of the colorectal cancer cell lines (HCT116, HCT116 p53---, HCT116 p21---) was not affected by B1R and B2R agonists. However, we observed by real-time PCR and western blot analysis a differential expression of B1R, B2R, and retinoid receptors transcripts and prot

روابط وملفات

التعريف والنوع

رقم الوثيقة

f22a5b2a-df34-47c9-8c23-3361fb8e1690

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل ماجيستير

نوع الملف

pdf text

أسماء الملفات

1634047_1.pdf

بيانات النشر

ألقاب المؤلفين

[{"name_ar":"Ayad Jaffa ","title_ar":"اشراف","title_en":"Supervision"},{"name_ar":"Celine Fouad Khati","title_ar":"اعداد","title_en":"Preparation"}]

اللغة

English

المصدر والدورية

اسم المصدر

Differential expression of the Bradykinin and Retinoic Acid Receptors in human colorectal cancer cell lines

المحتوى والصفحات

عدد الصفحات

0

إشراف وإعداد

الإشراف

Ayad Jaffa

الإعداد

Celine Fouad Khati

الاقتباسات الببليوغرافية