Expression and Purification of Glycosyltransferases in Pichia Pastoris: Towards Improving the Migration of Stem Cells by Enhancing Surface Expression of Sialyl Lewis X

الموضوعات

علوم بحتة وطبيعية

الملخص

Recruitment of circulating cells towards target sites is primarily dependent on E-selectin receptor/ligand adhesive interactions. Glycosyltransferase (GTs) are involved in the creation of E-selectin ligands. A sialofucosylated terminal tetrasaccharide like glycan structure known as sialyl Lewis x (sLex), is the most recognized ligand by selectins. This structure is found on the surface of cancer cells and leukocytes but is often absent on the surface of many adult stem cell populations. In order to synthesize sLex, GTs must be endogenously expressed and remain active within the cells. Generally, these stem cells express terminal sialylated lactosamine structures on their glycoproteins which require the addition of alpha-(1,3)-fucose to be converted into an E-selectin ligand. There are a number of fucosyltransferases (FUTs) that are able to modify terminal lactosamine structures to create sLex such as FUT6. In this work we focused on expressing and purifying active recombinant FUTs as a tool to help create sLex structures on the surface of adult stem cells in order to enhance their migration.

روابط وملفات

الوثيقة في المستودع الخارجي عرض في AskZad

التعريف والنوع

رقم الوثيقة: f2242266-3a20-4720-8d68-8837e1e70ed6
رقم العقد: 0
نوع الوسائط: Crawler
نوع المحتوى: الرسائل العلمية
صيغة المصدر: رسائل ماجيستير
نوع الملف: pdf text
أسماء الملفات: 1699467_1.pdf

بيانات النشر

ألقاب المؤلفين: [{"name_ar":"Asma Saeed Al-Amoodi","title_ar":"اعداد","title_en":"Preparation"},{"name_ar":"Jasmeen Merzaban ","title_ar":"اشراف","title_en":"Supervision"}]
اللغة: English

المصدر والدورية

اسم المصدر: Expression and Purification of Glycosyltransferases in Pichia Pastoris: Towards Improving the Migration of Stem Cells by Enhancing Surface Expression of Sialyl Lewis X

المحتوى والصفحات

عدد الصفحات: 0
كلمات الباحثين: Cellular migration , FUTs purification , Stem Cells

إشراف وإعداد

الإشراف: Jasmeen Merzaban
الإعداد: Asma Saeed Al-Amoodi

الاقتباسات الببليوغرافية

APA

Asma Saeed Al-Amoodi و Jasmeen Merzaban . (2017). Expression and Purification of Glycosyltransferases in Pichia Pastoris: Towards Improving the Migration of Stem Cells by Enhancing Surface Expression of Sialyl Lewis X. أطروحة(رسائل ماجيستير). جامعة الملك عبدالله للعلوم والتقنية. السعودية.

MLA

Asma Saeed Al-Amoodi و Jasmeen Merzaban . Expression and Purification of Glycosyltransferases in Pichia Pastoris: Towards Improving the Migration of Stem Cells by Enhancing Surface Expression of Sialyl Lewis X. 2017. جامعة الملك عبدالله للعلوم والتقنية، رسائل ماجيستير.