Pathological Evaluation of Staphylococcal Fatty Acid Modifying Enzyme (FAME) and Lipase on Abscess Production by Non- Abscess Producer Staphylococci in Sheep

الموضوعات

طب

الملخص

This work was carried out to explain the role of FAME and lipase insurvival and multiplication of the organism in the host tissues and theinduction of abscess by non abscess-producer Staphylococci treated withFAME and/or lipase and also to study the effect of triglycerides on abscessformation.Staphylococcus aureus (S. aureus)subsp. anaerobiusand S.epidermidiswere isolated from sheep abscess for extraction of FAME andlipase respectively by culturing themon trypticase soy broth medium.Thirty-nine sheep, divided into nine groups of three animals each,were inoculated subcutaneously (S/C) with different protocols and doses.Inocula included: S. epidermidisplus FAME; or plus FAME and lipase;S.warneriplus FAME or plus FAME and lipase; or plus lipase alone; S.aureus subsp. anaerobiusplus triglyceride; or plustriglyceride and lipase; orplus triglyceride and FAME. The control groups (six groups of two animalseach) were inoculated with the enzymes FAME and lipase, triglycerides andthe test bacteria (S. aureus subsp. anaerobius, S. epidermidis and S.warneri).Necropsy of the experimental sheep revealed the presence ofabscesses at the sites injected with S. epidermidisand S. warneriwhentreated with FAME or with both FAMEand lipase and also at sites where S.aureus subsp. anaerobiuswas inoculated. No abscesses were formed at sitesinjected with S. epidermidisor S. warnerialone or S. warneri plus lipase.Triglycerides were found to inhibit the formation of abscess when injectedwith abscess-producer Staphylococci (S. aureus subsp. anaerobius), butwhen FAME or lipase was also added, abscess was formed again.14Sections prepared from sites injected with bacteria and the enzymesFAME and lipase showed the presence of abscesses characterized byneutrophils infiltration surrounded by fibrous tissue reaction. At placeschronic inflammatory cells (mainly macrophages), fibroplasia andpyogranulomas were seen. The tissue changes were more pronounced atsites where S. epidermidiswas injected with FAME and lipase, followed byinoculation sites of S. epidermidisplus FAME, and S. aureus subsp.anaerobiusalone or when triglycerides were added to it with FAME orlipase. Sites injected with S. aureus subsp. anaerobiusplus triglyceridesshowed slight inflammatory reaction. To the best of our knowledge this in vivo experimental study seems tobe the first of its type.

روابط وملفات

التعريف والنوع

رقم الوثيقة

f1cf3de2-09ff-442b-99b9-7830fbf57613

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل دكتوراة

نوع الملف

pdf text

أسماء الملفات

1638240_1.pdf

بيانات النشر

ألقاب المؤلفين

[{"name_ar":"Ahmed Abdel Rahim Gameel","title_ar":"اشراف","title_en":"Supervision"},{"name_ar":"Raga Ibrahim Omar Ahmed ","title_ar":"اعداد","title_en":"Preparation"},{"name_ar":"Sulieman Mohamed El-Sanousi ","title_ar":"اشراف","title_en":"Supervision"}]

اللغة

English

المصدر والدورية

اسم المصدر

Pathological Evaluation of Staphylococcal Fatty Acid Modifying Enzyme (FAME) and Lipase on Abscess Production by Non- Abscess Producer Staphylococci in Sheep

المحتوى والصفحات

عدد الصفحات

0

إشراف وإعداد

الإشراف

Ahmed Abdel Rahim Gameel, Sulieman Mohamed El-Sanousi

الإعداد

Raga Ibrahim Omar Ahmed

الاقتباسات الببليوغرافية