Study the interaction of Bovine Serum Albumin with Vitamin C and Vitamin B12 Using Spectroscopic Techniques

الموضوعات

علوم بحتة وطبيعية

الملخص

Vitamin C (ascorbic acid) and Vitamin B12(Cobalamin) are belong to a hydrophilic vitamins which carried by blood proteins. Bovine serum albumin (BSA) is the major soluble protein constituent of the circulatory system and has many physiological function including transport of a variety of a compound.Binding of vitamin C and vitamin B12to bovine serum albumin (BSA) has been studied using Fluorescence spectroscopy,Ultraviolet-Visible (UV-Vis)absorption spectroscopy, and Fourier transform infrared (FT-IR) spectroscopy using constant protein concentration and various vitamins concentrationsat pH 7.4.UV-Visspectroscopywasused to analyze vitamin C and vitamin B12binding modes, the binding constant and the effect of the drug complexation on BSA stability and conformation, from UV-Vis spectral determined that the binding constant of vitamin C and B12with interaction of BSA (1.39×104M-1and 1.61×104M-1, respectively)Fluorescence spectroscopywas used to analyze the emission spectra of BSA in the absence and presence of vitamin C and vitamin B12, and to determine the Stern-Volmer quenching constant and the bimolecular quenching constant is (4.78× 103L.mol-1and 4.78×1011L.mol-1.s-1, respectively) for vitamin C and (2.42×104L.mol-1and 2.42×1012L.mlo-1.s-1, respectively) for vitamin B12. In addition, determined the values of bindingconstant from the Lehrer equation for binding of vitamin C and vitamin B12with BSA complex which are equals (1.6×104M-1and 1.44×104M-1, respectively).Where FT-IR was used to determine the protein secondary structure, which show a change in the secondary structureof BSA after interacting with vitamin C and vitamin B12.

روابط وملفات

التعريف والنوع

رقم الوثيقة

efb13545-a76c-4c20-b662-df418b34674e

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل ماجيستير

نوع الملف

pdf text

أسماء الملفات

1696633_1.pdf

بيانات النشر

ترجمة العنوان

دراسة التفاعل بين بروتين الالبومين البقري مع فيتامين C وفيتامين B12 باستخدام تقنيات اجهزة التحليل الطيفي.

ألقاب المؤلفين

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اللغة

English

المصدر والدورية

اسم المصدر

Study the interaction of Bovine Serum Albumin with Vitamin C and Vitamin B12 Using Spectroscopic Techniques

المحتوى والصفحات

عدد الصفحات

0

إشراف وإعداد

الإشراف

حسين سمامرة

الإعداد

اسراء ابراهيم محمود ابو سرور

الاقتباسات الببليوغرافية