Constituents, Antimicrobial and Antioxidant Activity of Oils from Selected Plant Species

الموضوعات

علوم بحتة وطبيعية

الملخص

The oils from targeted plant spies were extracted by n-hexane. Gas chromatography - mass spectrometry has been used for the identification and quantification of the studied oils. The extracted oils have been screened for Antibacterial Activity against five standard human pathogens. Citrullus colocyn this L. seed oil was characterized by GC-MS Revealed the presence of (22) components. Major constituents are : 9,12-Octadecadienoic acid (Z,Z)-, methyl ester (46.30 % ), Hexadecanoic acid, methyl ester (16.35% ) , 9-Octadecenoic acid (Z)-, methyl ester (15.58% ) Methyl stearate (14.34% ) .Pimpinella anisum L. seeds oil was characterized by GC-MS Revealed the presence of (24) components. Major constituents are Anethole (82.75%) , Butanoic acid 2-methyl-, 2- methoxy-4-(2- propenyl) phenyl ester (6.25 %).Melissa officinalis L. seeds oil was studied by GC-MS. The analysis revealed (26) constituents. Major constituents are:9,12- Octadecadienoic acid (Z,Z)-, methyl ester (52.94% ) - Octadecenoicacid (Z)-, methyl ester (12.01% ) , Hexadecanoic acid, methyl ester (11.25% ) and Methyl stearate (5.19% ) .`Origanum majarana L. seeds oil showed the presence of (35) constituents dominated by: 9,12,15-Octadecatrienoic acid, methyl ester, (Z,Z,Z) (40.78% ) 9,12-Octadecadienoic acid (Z,Z)-, methyl ester (33.61% ) , Hexadecanoic acid, methyl ester (11.00 %) and Methyl stearate (5.72 % ) .Raphanus sativus L. seeds oil gave total of (25) components major components are:13-docosenoic acid, methyl ester, (Z)-(28.02%),cis-13-eicosenoic acid, methyl ester(15.35%) , 9,12- octadecadienoic acid -(Z)-, methyl ester (12.75%) and hexadecanoic acid, methyl ester (8.63%).Lepidium sativum oil showed total of (25) constituents .Major constituents are:cis-13-eicosenoic acid methyl ester (16.48% ), 9,12-octadecadienoic acid (Z,Z)-, methyl ester(15.90 % ) , hexadecanoic acid methyl ester(13.11%) and 9,12,15-octadecatrienoic acid methyl ester(Z,Z,Z)-(10.16%).Citrullus colocynthis L. seed At a concentration of 100mg/ml Oil was inactivate against Bacillus subtilis, partially active against Staphylococcus aureus, good against Pseudomonas aeruginosa and partially active against Escherichia coli. The oil failed to give any Antibacterial Activity against Candida albicans. .Pimpinella anisum L. seeds oil at a concentration of 100mg/ml the oil showed activate against Bacillus subtilis, partially active against Staphylococcus aureus and Pseudomonas aeruginosa and very activate against Escherichia coli. The oil failed to give any Antibacterial Activity against Candida albicans. Melissa officinalis L. seeds oil exhibited good activate against. Staphylococcus aureus, Pseudomonas aeruginosa Candida albicans and Escherichia coli. The oil failed to give any Antibacterial Activity against Bacillus subtilis. Origanum majarana L. seeds oil showed partially activate against, Staphylococcus aureus and Pseudomonas aeruginosa, Candida albicans and Escherichia coli. The oil failed to give any Antibacterial Activity against Bacillus subtilis. In the DPPH assay Raphanus sativeus oil showed IC50: 68.65±1.23 close to that of the standard antioxidant: butylated hydroxyl toluene (BHT) – (IC50 61.11±1.78). In the superoxide anion scavenging assay , the oil sample gave : IC50 123.19±7.63 while the positive controls showed : BHT(IC50, 60.5±0.22) and Trolox (IC50 54.98±0.17).However , in the metal chelating assay the oil showed IC50: 132.53±0.80) .The antioxidant activity of Lepidium sativum oil was conducted. The oil showed a free radical scavenging capacity in the DPPH assay of: (IC50 97.36±0.9). In the metal chelating assay, the studied oil showed IC50: 118.25±0.31. In the superoxide anion scavenging assay, the oil sample showed ( IC50 11.72±0.17) .

روابط وملفات

التعريف والنوع

رقم الوثيقة

ef4480cd-e43e-4905-b7ac-5baa3454704d

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل دكتوراة

نوع الملف

pdf text

أسماء الملفات

2188081_1.pdf

بيانات النشر

ترجمة العنوان

المكونات ومضادات الميكروبات ونشاط مضادات الاكسدة لزيوت بعض انواع النباتات المختارة

ألقاب المؤلفين

[{"name_ar":" Mohammed Abdelkarim","title_ar":"اشراف","title_en":"Supervision"},{"name_ar":"Salwa Abdalla Ateem Aboanga ","title_ar":"اعداد","title_en":"Preparation"}]

اللغة

English

المصدر والدورية

اسم المصدر

Constituents, Antimicrobial and Antioxidant Activity of Oils from Selected Plant Species

المحتوى والصفحات

عدد الصفحات

0

ترجمة الملخص

تمت دراسة الزيوت المستخلصة من بعض النباتات بواسطة الهكسان .والتعرف على مكوناتها الكيميائية باستخدام كروماتوغرافياالغاز –طيف الكتلة , كما تم تحديد نشاطها الحيوي ضد خمسة انواع من الميكروبات المسببة للامراض للانسان .

كلمات الباحثين

Antimicrobial Antioxidant Activity Oils from Plant Species

إشراف وإعداد

الإشراف

Mohammed Abdelkarim

الإعداد

Salwa Abdalla Ateem Aboanga

الاقتباسات الببليوغرافية