Développement d’un biocapteur optique à tyrosinase de truffe (Terfezia leonis Tul.) immobilisée sur gel de silice pour le dosage du catéchol en solution

الموضوعات

علوم بحتة وطبيعية

الملخص

Catechol is widely used in many manufacturing industry, but has been recognized as a toxic andcarcinogenic to humans and animals. Therefore, it is necessary to have a sensitive, less expensive, simpleand reproducible analytical method for catechol detection. Conventional analytical methods such asspectrophotometry, capillary electrophoresis, voltammetry, chromatography and chemiluminescence haveseveral disadvantages thus limiting their use for the determination of this pollutant. Actually, immobilizedtyrosinase biosensors have received a great attention in the analytical field for the determination of phenolsin solution. This work aims to develop an optical biosensor using a crude extract of desert truffle (TerfezialeonisTul.) as an enzymatic source of tyrosinase. The biosensor is constructed by the immobilization oftyrosinase crude extract in a bi-layered silica gel film prepared by dip-coating of an alkoxide/colloidal silicasolution containing the enzyme on glass slide. Encapsulation has a moderate effect of the enzyme optimal pHstability but largely increases its thermal stability. Immobilized enzymes have a higher substrate affinitytowards catechol (KMapp = 0,3mM) but smaller maximum conversion velocity (VMaxapp). The opticalbiosensor showed a good reproducibility and provides a linear response for catechol in the concentrationrange of 50–400 μM with a limit of detection 52 μM. AFM studies show that the enzymes affect the silicagel structure, preventing further deposition of additional layers. The optical biosensor with desert truffletyrosinase can be considered as a promising tool for catechol analysis in pharmaceutical and environmentalfields.

روابط وملفات

التعريف والنوع

رقم الوثيقة

eea06241-72f9-4a1d-865d-42c7d078fdf2

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل دكتوراة

نوع الملف

pdf text

أسماء الملفات

904032_1.pdf

بيانات النشر

ألقاب المؤلفين

[{"name_ar":"Hicham Gouzi","title_ar":"اشراف","title_en":"Supervision"},{"name_ar":"Saida Leboukh","title_ar":"اعداد","title_en":"Preparation"},{"name_ar":"Yahia Harek","title_ar":"اشراف","title_en":"Supervision"}]

اللغة

French

المصدر والدورية

اسم المصدر

Développement d’un biocapteur optique à tyrosinase de truffe (Terfezia leonis Tul.) immobilisée sur gel de silice pour le dosage du catéchol en solution

المحتوى والصفحات

عدد الصفحات

0

إشراف وإعداد

الإشراف

Hicham Gouzi, Yahia Harek

الإعداد

Saida Leboukh

الاقتباسات الببليوغرافية