Molecular Phylogeny of Palestinian Date Palm Cultivars by Microsatellite Simple Sequence Repeats and Inter Simple Sequence Repeats Markers

الموضوعات

علوم بحتة وطبيعية

الملخص

Date palm (Phoenix dactylifera L.) is considered one of the major fruit crops in Palestine and one of the oldest cultivated plants. Date crops contribute to the economical, industrial, and religious concepts in the Middle East and North Africa. Since studies on the genetic diversity and relatedness of date palm germplasm are limited in Palestine, the aim of this study is to analyze the genetic relatedness among twelve cultivars collected from the Jericho City area in Palestine using Simple Sequence Repeat (SSR) and Inter- Simple Sequence Repeat (ISSR). These cultivars are Zahidi, Hayani, Hejazi, Khadrawy, Barhy, Majool, Dejlet Noor (Deek Noor), Amery, Ibrahemi, and three cultivars known as Baladi.DNA was extracted and purified from the young leaves by applying a phenol-chloroform extract followed by DNeasy DNA extraction kit. Microsatellites (Simple Sequence Repeats; SSR) and Inter Simple Sequence Repeats (ISSR) were amplified by PCR using 3 SSR and 9 ISSR primers, respectively. The size of the amplicons of the SSR and ISSR products ranged between 180-240bp and 140 - 3100bp, respectively. A matrix representing the banding profiles was used to establish a phylogenetic tree of the twelve cultivars using the Fingerprint Analysis with Missing Data (FAMD) software and the Neighbor Joining method.The phylogram obtained by SSR and ISSR encompasses three clusters. The first cluster contained one cultivar Ameri. The second cluster included 5 cultivars, which was further divided into two sub-clusters; the first sub-cluster contained one cultivar; Baladi 3, and the second sub-cluster encompasses four cultivars, Ibrahimi, Khadrawi, Dejlat Noor and Hayani. The third cluster was divided into two sub-clusters; the first one contains Majool and Berhy, while the other one holds Balady 2, Zuhadi, Hijazi, and Baladi 1. The fidelity of the phylogram was assured by the bootstrapping and Principal Coordinate of Analysis (PCoA) methods.Despite the well-known morphological differences among some cultivars, they were closely related in this phylogram. Examples include Ibrahemy and Khadrawi, Dejlat Noor and Hayani, as well as Hijazi and Baladi 1. The phylogram differentiates among three cultivars that are known as Baladi. These three cultivars were named as Baladi1-3 to facilitate their use in this study. To the best of my knowledge, this is the first study done in Palestine with emphasis on the genetic relatedness among date palms cultivars using SSR and ISSR.

روابط وملفات

التعريف والنوع

رقم الوثيقة

ee936934-fbc5-43ab-8250-7c6576de7344

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل ماجيستير

نوع الملف

pdf text

أسماء الملفات

1819865_1.pdf

بيانات النشر

ترجمة العنوان

تحديد الطرز الوراثي لأهم أصناف النخيل في فلسطين باستخدام التسلسل البسيط المتكرر والمناطق التي تقع بين هذه التسلسلات

ألقاب المؤلفين

[{"name_ar":"Omar Darissa","title_ar":"اشراف","title_en":"Supervision"},{"name_ar":"هلا علي محمد الشولي","title_ar":"اعداد","title_en":"Preparation"}]

اللغة

English

المصدر والدورية

اسم المصدر

Molecular Phylogeny of Palestinian Date Palm Cultivars by Microsatellite Simple Sequence Repeats and Inter Simple Sequence Repeats Markers

المحتوى والصفحات

عدد الصفحات

0

كلمات الباحثين

Science

إشراف وإعداد

الإشراف

Omar Darissa

الإعداد

هلا علي محمد الشولي

الاقتباسات الببليوغرافية