Estimation the levels of (coenzyme Q10) and Respiratory Factor Erythroid 2–Related Factor 2 (Nrf-2) in Pre diabetes and type 2 diabetes mellitus Patients

الموضوعات

طب

الملخص

Type 2 Diabetes Mellitus (T2DM) and its precursor, pre-diabetes, are characterized by chronic oxidative stress and metabolic dysfunction. Coenzyme Q10 (CoQ10), a vital mitochondrial antioxidant, and Nuclear Factor Erythroid 2–Related Factor 2 (Nrf2), a master regulator of antioxidant defenses, are crucial in cellular protection. This study aimed to estimate the levels of serum CoQ10 and Nrf2 in T2DM patients, pre-diabetic individuals, and healthy controls, and to evaluate their diagnostic potential. Methods: A case control was conducted from September 2024 to July 2025 in Iraq , study was carried out at Imam Hassan Center for Endocrinology and Diabetes /Internal Medicine Consultant and Imam Al – Hassan Al Mujtaba teaching hospital in Kerbala city/ Internal Medicine Consultant. This study involved 88 individuals, including 46 type II diabetes patients , pre diabetes patients (12) and 30 volunteers as a control, with inclusion criteria including fasting blood sugar and haemoglobin A1c results. Blood samples were collected from both control and patient groups after 12 hours of fasting.Data were collected via a structured questionnaire covering sociodemographics, medical history, and lifestyle, complemented by clinical evaluations by specialist physicians. Serum lipid profile were measured using fully automatic chemistry analyzer (SMART-120, Geno TEK, United States of America). ELISA system was used for the detection of Nrf2 level. Q10levels were measured quantitatively by high-performance liquid chromatography. Results: The study groups were well-differentiated by HbA1c and lipid profiles, with DM and pre-DM groups exhibiting typical glycemic and dyslipidemic patterns. Serum CoQ10 levels showed a clear and significant progressive decline from healthy controls (median 1.6 U/L) to the pre-diabetic group (median 0.89 U/L) and lowest in DM patients (median 0.38 U/L). In contrast, median serum Nrf2 levels were remarkably similar and largely overlapping across all three groups (medians: Healthy 26, Pre-DM 30, DM 29), indicating no significant difference.ROC curve analysis revealed excellent diagnostic accuracy for CoQ10: an Area Under the Curve (AUC) of 0.9724 (p<0.0001) for differentiating DM patients from healthy controls, with an optimal cut-off of <1.275 U/L. For identifying pre-diabetic individuals, CoQ10 showed an AUC of 0.9013 (p<0.0001), with an optimal cut-off of <1.120 U/L. Correlation analysis revealed a significant positive correlation between CoQ10 and Nrf2 in both DM (r=0.4, p=0.05) and pre-DM (r=0.65, p=0.001) groups. Notably, CoQ10 was strongly negatively correlated with HbA1c in the pre-DM group (r=-0.920, p=0.027), and positively correlated with HDL in the DM group (r=0.332, p=0.005). Conclusion: Serum CoQ10 levels progressively decrease with the advancement of metabolic dysregulation and serve as a highly accurate biomarker for diagnosing both pre-diabetes and Type II Diabetes Mellitus. Its strong associations with glycemic control and lipid profiles highlight its integral role in metabolic health. Conversely, circulating Nrf2 levels do not appear to be a reliable diagnostic marker for these conditions. These findings underscore the potential of CoQ10 as a valuable non-invasive tool for screening and monitoring in the context of impaired glucose metabolism.

روابط وملفات

التعريف والنوع

رقم الوثيقة

eddb9f2a-1555-4561-97a1-2c9a081b03c8

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل ماجيستير

نوع الملف

pdf text

أسماء الملفات

1085705_1.pdf

بيانات النشر

ترجمة العنوان

تقدير مستويات الانزيم المساعد (Co Q10) والعامل النووي المرتبط بكريات الدم الحمراء 2(Nrf2) في المرضى المصابين بداء السكري من النوع الثاني

ألقاب المؤلفين

[{"name_ar":"اثير حميد عوده ","title_ar":"اشراف","title_en":"Supervision"},{"name_ar":"حسن حيدر خضر","title_ar":"اشراف","title_en":"Supervision"},{"name_ar":"حيدر عبد الحسين طاهر","title_ar":"اعداد","title_en":"Preparation"}]

اللغة

English

المصدر والدورية

اسم المصدر

Estimation the levels of (coenzyme Q10) and Respiratory Factor Erythroid 2–Related Factor 2 (Nrf-2) in Pre diabetes and type 2 diabetes mellitus Patients

المحتوى والصفحات

عدد الصفحات

0

إشراف وإعداد

الإشراف

اثير حميد عوده , حسن حيدر خضر

الإعداد

حيدر عبد الحسين طاهر

الاقتباسات الببليوغرافية