The Prevalence of Human Papilloma (type 16 & 18) and Epstein-Barr viruses in oral squamous cell carcinoma and their association with other risk factors in some Yemeni patients (2009-2012)

الموضوعات

طب

الملخص

Prevalence of Human Papilloma (type 16 & 18) and Epstein-Barr viruses inoral squamous cell carcinoma and their association with other risk factors insome Yemeni patients (2009-2012)Background: It is generally agreed that tobacco and alcohol consumption are themajor risk factors for developing oral squamous cell carcinoma (OSCC). However,some patients develop OSCC without exposure to these risk factors. This factsuggests that additional causes, such as oncogenic viruses, may also help cells todevelop OSCC.Objective: The aim of this study is to determine the prevalence of the high-riskhuman papilloma viruses– types 16 and 18- and Epstein-Barr Virus in someYemeni patients with oral squamous cell carcinoma and their association withother various risk factors.Material and methods: The study was carried at Sana`a- Al-Thawra ModernGeneral Hospital and Al-Gomhory Teaching Hospital, in the period June 2009 -March 2011.The material of the study consisted of 60 patients with oral squamouscell carcinoma referred for management, and 120 control cases matched for genderand age. Data including demographic variables, chewing qat - the fresh leaves andyoung shoots of the plant Catha edulis forsks-, smoking tobacco, alcohol drinkingand shammah use – a complex smokeless tobacco mixture consisting of powderedtobacco leaf, slaked lime (calcium carbonate), ash, oil and other substances, e.g.black pepper, mint, and flavours - were recorded. The cases were examined for thesite and side affected by cancer and each case was clinically staged. Fresh biopsieswere taken from the case group, and buccal cells samples were collected by aswish and spit protocol from the controls. For the purpose of comparison, andchecking the validity and efficacy of the PCR methodology, five paraffinXVembedded tissue blocks specimens of cervical squamous cell carcinomas weretested using a viral PCR assay. Histological analysis was done for the cancerbiopsies. DNA extraction was done for both groups samples.Results: Taqman real-time Polymerase chain reaction (q-PCR) was done forexamination of type 16 and 18 human papilloma viruses and Epstein-Barr virus .Viral q-PCR assays achieved an excellent linearity (R2>0.99) over a dynamicrange of 10 to106 copies allowing determination of as little as 200 viral copies /sample. Neither type 16 nor type 18 of HPV was found in any of the cases or thecontrols. HPV type 16 and 18 were detected in all the five cases of cervical cancer(100%). EBV was detected in 73.3% and 60.8% of the cases and controls,respectively; and the difference was statistically insignificant. There was nocorrelation or interaction between the presence of EBV and any demographic orrisk factors. Putting all the risk factors as co-variates in a logistic regressionmodel, EBV showed borderline association with oral cancer with an odd ratio of2.58, Shammah use was found to be the only determinant risk factor for oralcancer with odd ratios of 11.0 and 54.0 for the ex-user and current user,respectively. Surprisingly, ex-smoker emerged as a protective factor.Conclusion: the result of this study showed that:1/ Neither HPV nor EBV, has a relation with oral squamous cell carcinoma.2/ There was a strong association between Shammah use and oral cancer.

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التعريف والنوع

رقم الوثيقة

eceeac6a-7193-4970-b13f-b8598b99655a

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل دكتوراة

نوع الملف

pdf text

أسماء الملفات

1639965_1.pdf

بيانات النشر

ألقاب المؤلفين

[{"name_ar":"أحمد سليمان ","title_ar":"اشراف","title_en":"Supervision"},{"name_ar":"أكرم ثابت محسن نشار","title_ar":"اعداد","title_en":"Preparation"}]

اللغة

English

المصدر والدورية

اسم المصدر

The Prevalence of Human Papilloma (type 16 & 18) and Epstein-Barr viruses in oral squamous cell carcinoma and their association with other risk factors in some Yemeni patients (2009-2012)

المحتوى والصفحات

عدد الصفحات

0

إشراف وإعداد

الإشراف

أحمد سليمان

الإعداد

أكرم ثابت محسن نشار

الاقتباسات الببليوغرافية