Molecular typing of Candida albicans isolates from Lebanese hospitals in correlation to: Phenotypic identification, epidemiology, antifungal resistance profile, and biofilm formation

الموضوعات

علوم بحتة وطبيعية

الملخص

Candida species are the most common opportunistic pathogens in debilitated or immunocompromised hosts and cause systemic candidiasis, which has high rates of morbidity and mortality. Most of these infections are nosocomial and raise the issue of hospital hygiene. As such the identification and subtyping of Candida species is epidemiologically important for recognizing outbreaks of infection, detecting cross-transmission, determining the source of the infection, and recognizing particularly virulent strains, or detecting the emergence of drug resistant strains. In this study our objective is to compare Candida albicans hospital identification rates with conventional laboratory employed methods such as API, CHRO Magar and germ tube formation and a more reliable molecular method such as ITS typing. Furthermore our aim was to assess biofilm-forming capacities with drug resistance profiles and correlate them with MLST typing of isolates. ITS typing was performed for 75 isolates and the misidentification rate for each method is considered significant: 9.3% for API and CHRO Magar, and17.3% for the hospital. Moreover, antifungal susceptibility testing was performed for 4 antifungal drugs encompassing different antifungal classes, and antifungal resistance to3 and to the 4 drugs was found to occur amongst 25% of isolates tested which was unexpected and raises serious questions concerning the methods of treatment.Interestingly some strains with significant biofilm forming capabilities correlated well with strains that were multidrug resistant and such strains grouped closely together in a neighbor-joining tree generated by MLST typing indicating phylogenetic relatedness or strain microevolution or recurrent infection. In conclusion, this pilot study gives insight about many aspect of Candida albicans isolates circulating in hospitals, unfortunately the small sample size and lack of information about patient history, preclude any firm conclusions.

روابط وملفات

التعريف والنوع

رقم الوثيقة

ec75d191-0bed-41b9-ac5a-64a4b9c653a1

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل ماجيستير

نوع الملف

pdf text

أسماء الملفات

2213287_1.pdf

بيانات النشر

ألقاب المؤلفين

[{"name_ar":" Ibrahim Emmad Bitar","title_ar":"اعداد","title_en":"Preparation"},{"name_ar":" Roy Khalaf","title_ar":"اشراف","title_en":"Supervision"}]

اللغة

English

المصدر والدورية

اسم المصدر

Molecular typing of Candida albicans isolates from Lebanese hospitals in correlation to: Phenotypic identification, epidemiology, antifungal resistance profile, and biofilm formation

المحتوى والصفحات

عدد الصفحات

0

كلمات الباحثين

biofilm formation

إشراف وإعداد

الإشراف

Roy Khalaf

الإعداد

Ibrahim Emmad Bitar

الاقتباسات الببليوغرافية