Enhancement of Sporulation of Madurella mycetomatis using Natural Substrates and Synthetic Media

الموضوعات

علوم بحتة وطبيعية

الملخص

The fungus Madurella mycetomatis is the common causative agent ofmycetoma in Sudan and worldwide. The disease represents a mycologicalhealth problem with poor mycological diagnosis. Study of sporulation ofM. mycetomatis assists in the identification of the fungus, and helps in thestudy of its natural habitat and any possible survival structures. In thisstudy, 25 fungal isolates from black grain eumycetoma patients wereincluded. The isolates were specifically identified as M. mycetomatisusing species-specific PCR. Media used to enhance the sporulation were:Corn Meal agar, Water agar, Soil agar, Czapek dox agar, and Ascosporeagar. In all these media all clinical isolates grew well, while in Ascosporeagar only 14 out of the 25 isolates were able to grow. All types of media(except Ascospore agar) supported asexual sporulation of M.mycetomatis, at least in half of the isolates. In Corn Meal agar 13 out ofthe 25 isolates sporulated, and in Czapek dox agar 12 isolates sporulated.In Water and Soil agar 10 isolates were able to sporulate.vThe spores (conidia) were small obovoidal, rounded to pyriform. Theseconidia were formed on the tip of hyaline well differentiatedconidiophores. More than one conidium can be produced from singleconidiophore. The mode of conidiogenesis was basipetal non-catenatedphialidic enteroblastic conidiogenesis.Some M. mycetomatis isolates were able to produce in vitro grains(sclerotia). Sclerotia were commonly formed on Corn Meal agar. In thismedium, 14 out of the 25 isolates produced sclerotia. On Czapek dox agar7 isolates produced sclerotia, and 3 isolates in Soil agar formed sclerotia,while only 2 isolates formed sclerotia in Water agar.In conclusion, the best medium for sporulation was found to be CornMeal agar. Czapek dox agar can be used as a medium for growth andsporulation of M. mycetomatis, but Ascospore agar was not a suitablemedium for M. mycetomatis growth and sporulation.

روابط وملفات

التعريف والنوع

رقم الوثيقة

eb9eac67-650a-41fe-87f7-8e7cffe3d733

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل ماجيستير

نوع الملف

pdf text

أسماء الملفات

1640085_1.pdf

بيانات النشر

ألقاب المؤلفين

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اللغة

English

المصدر والدورية

اسم المصدر

Enhancement of Sporulation of Madurella mycetomatis using Natural Substrates and Synthetic Media

المحتوى والصفحات

عدد الصفحات

0

إشراف وإعداد

الإشراف

الشيخ محجوب جعفر

الإعداد

سارة عبدالله أحمد إبراهيم

الاقتباسات الببليوغرافية