Genetic Polymorphisms of Glutathione S-transferase GSTM1 and GSTT1 among Acute Myeloid Leukemic Patients, National Cancer Institute, University of Gezira, Sudan (2018)

الموضوعات

طب

الملخص

Leukaemias in general population result from interaction between genetic and environmental factors and time. The interplay of xenobiotics exposure, endogenous physiology, and genetic variability of multiple loci may facilitate knowledge about leukaemia etiology and the identification of individuals who are at increased risk of developing leukaemias. This is hospital based case control study aimed to determine the genetics polymorphism of GSTT1 and GSTM1 as risk factor to develop acute myeloid leukemia. The sample were collected from diagnosed patients who attended the National Cancer Institute –Gezira state during the period of (2017 -2018). The study included: 20 cases and 20 healthy volunteers matched for sex and age whose were selected according to inclusion and exclusion criteria .Three ml of blood was collected in EDTA tubes, then the complete blood count (CBC) was done by automated hematology analyzer and followed by DNA extraction and then amplification by PCR using predesigned primers the products visualized by using gel documentation system. Data analysis was done by statistical package for social science computer program. The study results showed the frequency of null, GSTM1 and GSTT1 deletion genotype was 55%, 20% and 15%, respectively in AML patient where as 30%, 15% and 45% respectively in controls. No statistical significant differences in GSTM1 deletion, GSTT1 deletion and null genotypes between AML patients as compared to controls (p-value 0.202, 0.189 and 0.582) retrospectively .Also there was no statistical significant among different age groups and genders (p.value 0.704and 0.269) retrospectively. The CBC parameters show highly statistical significant difference between the cases and controls (p.value 0.00) except the WBCs show no statistical significant difference between cases and control (p.value 0.064). The Study recommended that use advance technique for future screening and involving other GST family among different age groups, and use this polymorphism as prognostic marker and chemotherapy response marker. The study recommended that use advance technique for future screening and involving other GST family among different age groups.

روابط وملفات

التعريف والنوع

رقم الوثيقة

eb6a49fb-3647-4520-9982-0b6d275577fb

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل ماجيستير

نوع الملف

pdf image

أسماء الملفات

eb6a49fb-3647-4520-9982-0b6d275577fb_1.pdf

بيانات النشر

ترجمة العنوان

تعدد أشكال الوراثة من GSTT1 و GSTM1 كعامل خطر لزيادة حدوث سرطان الدم النخاعي الحاد - المعهد القومى للسرطان جامعة الجزيرة - السودان (2018)

ألقاب المؤلفين

[{"name_ar":" Salah Alzaki","title_ar":"اشراف","title_en":"Supervision"},{"name_ar":"Ozaz Yagoub ","title_ar":"اشراف","title_en":"Supervision"},{"name_ar":"مصطفى إبراهيم عباس البشير ","title_ar":"اعداد","title_en":"Preparation"}]

اللغة

English

المصدر والدورية

اسم المصدر

Genetic Polymorphisms of Glutathione S-transferase GSTM1 and GSTT1 among Acute Myeloid Leukemic Patients, National Cancer Institute, University of Gezira, Sudan (2018)

المحتوى والصفحات

عدد الصفحات

0

إشراف وإعداد

الإشراف

Salah Alzaki, Ozaz Yagoub

الإعداد

مصطفى إبراهيم عباس البشير

الاقتباسات الببليوغرافية