Development and Validation of Spectrophotometric Method for Determination of Gabapentin and Gentamicin in Bulk and Pharmaceutical Dosage Forms

الموضوعات

طب

الملخص

AbstractA sensitive, simple, accurate and economic spectrophotometric method has been developed for the determination of gabapentin (GBP) and gentamicin (GEN) in pure form and pharmaceutical preparations. The method is based on Schiff base formation through condensation reaction of the primary amino group of GBP and GEN with an active carbonyl group of salicylaldehyde reagent (SA) in presence of acetate solution. The obtained yellow coloured derivatives in methanolic medium showed absorption maxima at 403 nm and 416 nm, for GBP and GEN, respectively and they were found to be stable at least for 1 hour after dilution. Different variables affecting the reactions were studied and optimized such as the type of solvent, reagents concentration, pH effect, reaction temperature and reaction time. Under the optimum conditions, the absorbance was found to increase linearly with increasing concentration with correlation coefficient values of 0.9961 and 0.994 for GBP and GEN, respectively. The validity of the described method was assessed according to the ICH guidelines. Beer's law was obeyed in the concentration ranges of 6 to 100 ?g/mL and 13.6 to 170 ?g/mL for GBP and GEN, respectively. Limit of detection (LOD) and limit of quantification (LOQ) were found to be 4.24 and 12.84 ?g/mL for GBP and 8.37 and 25.35 ?g/mL for GEN, respectively. The mean percentage recoveries ± standard deviation (SD) (n = 9) were 100.57 ± 1.5 and 100.88 ± 1.02 by applying the standard addition technique for GBP and GEN, respectively. The method was precise (relative standard deviation (RSD) ? 1.34% and ? 1.23% for GBP and GEN, respectively). It was successfully applied for determination of the investigated drugs in their pharmaceutical dosage forms without detectable interference from the additives commonly present in the pharmaceutical preparations. Statistical comparison of the results was performed using t-test at 95% confidence level, and there was no significant difference between the official and the proposed method with regard to accuracy, hence can be suggested for routine analysis of both drugs.Keywords: Derivatization, gabapentin, gentamicin, salicylaldehyde, spectrophotometric.

روابط وملفات

التعريف والنوع

رقم الوثيقة

eb69887a-4b54-400a-b701-05b55563456b

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل ماجيستير

نوع الملف

pdf text

أسماء الملفات

2102867_1.pdf

بيانات النشر

ترجمة العنوان

تطوير وتحقق من طريقة تحليل طيفي لتحديد كمية دوائي الاجابابنتين والجنتاميسين في المادة الخام والاشكال الصيدلانية

ألقاب المؤلفين

[{"name_ar":"Eman Hussein Abd Algharably","title_ar":"اعداد","title_en":"Preparation"},{"name_ar":"إيهاب مصطفي المصري","title_ar":"اشراف","title_en":"Supervision"},{"name_ar":"مي عبد الرحمن رمضان","title_ar":"اشراف","title_en":"Supervision"}]

اللغة

English

المصدر والدورية

اسم المصدر

Development and Validation of Spectrophotometric Method for Determination of Gabapentin and Gentamicin in Bulk and Pharmaceutical Dosage Forms

المحتوى والصفحات

عدد الصفحات

0

إشراف وإعداد

الإشراف

إيهاب مصطفي المصري, مي عبد الرحمن رمضان

الإعداد

Eman Hussein Abd Algharably

الاقتباسات الببليوغرافية