Recherche et évaluation des activités biologiques de trois extraits d’Helix aspersa (aqueux, hydro alcoolique et organique) Activités anti-inflammatoire, anti tumorale et anti-angiogénique

الموضوعات

علوم بحتة وطبيعية

الملخص

Although the field of biotechnology has developed extraordinarily in recent years, therapiesremain ineffective and can cause side effects in patients, which is why the laboratories have turned to the search for new natural products. Helix aspersa is a terrestrial mollusk that is widespread in Mediterranean countries and is part of the food habitude. It is an important source of protein and mineral salts and has several therapeutic effects known for centuries.One of the main prospects of our study is the evaluation of the anti-inflammatory, anti-tumor and anti-angiogenic effects. Three extracts were prepared using solvents with differentpolarity: an aqueous extract, an hydroalcoholic extract and an organic extract. Then, a modelof acute pulmonary inflammation was developed to evaluate in vivo the anti-inflammatoryactivity of extracts by measuring total white blood cells and white blood cells inbronchoalveolar fluid, myeloperoxydase activity and analyzing the histology of pulmonarytissue. Analysis of the results showed that the extracts had anti-inflammatory activity bydecreasing the total leukocyte count in blood and in bronchoalveolar fluid, protecting thepulmonary tissue by modulating neutrophil activity and decreasing protein concentration inbronchoalveolar fluid. The antiproliferative activity of the extracts was evaluated by trypanblue and the MTT test on several cell lines: MK2, A549, HUH7, K562, HUT-78 and SeAx.The chromatin condensation and nuclear fragmentation were evaluated by the Hoechst test inthe HUT-78 and SeAx tumor cell line stimulated by the aqueous extract. The aqueous andhydroalcoholic extracts had antiproliferative activity against the HUT-78 and SeAx lines. Theaqueous extract induced apoptosis in HUT-78 cells. The hydroalcoholic and organic extractshad an antiproliferative activity on the HUH7 line. The three extracts were not toxic on theMK2 normal cell line. The antiangiogenic effect was evaluated by measuring the expressionof MMP-9 in the two HUT-78 and SeAx lines stimulated in the presence of the aqueous andhydroalcoholic extracts using was the zymography method. The aqueous and hydroalcoholicextracts had no effect on the production of MMP-9. At the end, we extracted the solubleproteins from meat lyophilized and we fractionated the extracts by anions exchangechromatography. The proteolytic activity using synthetic substrates and protease inhibitors aswell as the antiproliferative activity of the different fractions obtained were measured. One ofthe fractions contains proteases and has antiproliferative activity against the K562 liquid line.Nevertheless, other contained no enzymes and had a toxic effect on the matrix tumor linesA549 and HUH7. It was concluded that the aqueous, hydroalcoholic and organic extracts ofHelix aspersa had an anti-inflammatory and an anti-tumor effects, but did not have an antiangiogenic effect (on the expression of MMP-9)

روابط وملفات

التعريف والنوع

رقم الوثيقة

101495f0-8b10-4117-9b5b-495064329eaa

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل دكتوراة

نوع الملف

pdf text

أسماء الملفات

796104_1.pdf

بيانات النشر

ترجمة العنوان

Activités anti-inflammatoire, anti tumorale et anti-angiogénique

ألقاب المؤلفين

[{"name_ar":" Romeila Mebirouk","title_ar":"اعداد","title_en":"Preparation"},{"name_ar":"Dalila Naimi","title_ar":"اشراف","title_en":"Supervision"}]

اللغة

French

المصدر والدورية

اسم المصدر

Recherche et évaluation des activités biologiques de trois extraits d’Helix aspersa (aqueux, hydro alcoolique et organique) Activités anti-inflammatoire, anti tumorale et anti-angiogénique

المحتوى والصفحات

عدد الصفحات

0

إشراف وإعداد

الإشراف

Dalila Naimi

الإعداد

Romeila Mebirouk

الاقتباسات الببليوغرافية