Antimicrobial Susceptibility and Molecular Detection of Carbapenem Resistant Genes NDM-1 and IMP-1 of Pseudomonas aeruginosa Isolated from Clinical Specimens in Khartoum State-Sudan

الموضوعات

طب

الملخص

Carbapenems are the most powerful β-lactam antibiotics against bacteria especially multidrug resistant isolates like Pseudomonas aeruginosa (P.aeruginosa). This study was conducted to detect carbapenem resistance genes of P. aeruginosa from clinical specimens in Khartoum State in period from January _ June).A total of Fivety (n=50) isolates of p. aeruginosa were included in this descriptive cross sectional laboratory based study. P aeruginosa were isolated from urine, blood, wound swab , sputum and Body fluids samples, which were obtained from different hospitals in Khartoum State. The samples were collected from both males and females with different age using non-self-constructing information list. The isolated organism was stored in 20% Glycerol peptone media and was inoculated on CLED agar. Antibiotic susceptibility tests were carried out using Kirby Bauer technique. DNA was extracted using boiling method. The isolates were tested for the presence of carbapenem resistance genes (NDM, IMP) using Multiplex PCR.Out of 50 P. aeruginosa isolates, 19 (38%) were positive for one or both carbapenem resistance genes; 6 (12%) were positive for NDM gene and 13(26%) were positive for IMP gene. Tow isolates possess both genes.The study concluded that, there was a high percentage and multiplicity of carbapenem resistance genes among P. aeruginosa isolates in Khartoum State.

روابط وملفات

التعريف والنوع

رقم الوثيقة

1010db11-03f6-4a9a-b8f4-e281a6780049

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل ماجيستير

نوع الملف

pdf text

أسماء الملفات

2442720_1.pdf

بيانات النشر

ترجمة العنوان

اختبار حساسية المضادات الحيوية والكشف الجزيئي لجينات مقاومة الكاربابينيم ان دي ام و اي ام بي في الزائفه الزنجاريه المعزولة من عينات سريرية في ولایة الخرطوم- السودان

ألقاب المؤلفين

[{"name_ar":" Aisha Makawi Mohamed","title_ar":"اعداد","title_en":"Preparation"},{"name_ar":"Mutaz Fathelrahmansaad Hamad","title_ar":"اشراف","title_en":"Supervision"}]

اللغة

English

المصدر والدورية

اسم المصدر

Antimicrobial Susceptibility and Molecular Detection of Carbapenem Resistant Genes NDM-1 and IMP-1 of Pseudomonas aeruginosa Isolated from Clinical Specimens in Khartoum State-Sudan

المحتوى والصفحات

عدد الصفحات

0

كلمات الباحثين

Antimicrobial Susceptibility Carbapenem Resistant Genes Pseudomonas aeruginosa

إشراف وإعداد

الإشراف

Mutaz Fathelrahmansaad Hamad

الإعداد

Aisha Makawi Mohamed

الاقتباسات الببليوغرافية