دراسة على المستوي الجزيئي لتبدلات التعبير عن الجينات المرمزة لبروتينات PSA بين الطور المخمج Metayclic Promastigote والطور غير المخمج Procyclic Promastigote لطفيلي اللشمانيا الجلدية L. major

الموضوعات

علوم بحتة وطبيعية

روابط وملفات

التعريف والنوع

رقم الوثيقة

0f8ac892-76e2-488a-ad46-8ff9a6d822cd

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل ماجيستير

نوع الملف

pdf text

أسماء الملفات

1966551_1.pdf

بيانات النشر

ألقاب المؤلفين

[{"name_ar":"شادي سكرية","title_ar":"اشراف","title_en":"Supervision"},{"name_ar":"عبد الرحمن يوسف","title_ar":"اعداد","title_en":"Preparation"},{"name_ar":"محمود قويدر","title_ar":"اشراف","title_en":"Supervision"}]

اللغة

Arabic

المصدر والدورية

اسم المصدر

دراسة على المستوي الجزيئي لتبدلات التعبير عن الجينات المرمزة لبروتينات PSA بين الطور المخمج Metayclic Promastigote والطور غير المخمج Procyclic Promastigote لطفيلي اللشمانيا الجلدية L. major

المحتوى والصفحات

عدد الصفحات

127

ترجمة الملخص

Leishmaniasis is a neglected disease caused by the protozoan parasite belongs to the genus Leishmania and transmitted by the bite of an infective sand fly. Leishmania proliferates in the sand fly gut as a flagellated noninfectious form (Procyclic Promastigote) and later differentiates into the infectious Metacyclic Promastigote form while in vertebrate hosts it proliferates intracellularly as an a flagellated form )Amastigotes(after infection of macrophages. To understand host-parasite interactions at the molecular level, we have to focus on the macromolecules present at the surface of the parasite such as Promastigote Surface Antigens (PSA). In L .major, PSA is a family of 32 PSA genes encoding either secreted or membrane-anchored surface proteins. These genes are dispersed on chromosomes 5, 9, 12, 21 and 31. Only a few genes belonging to the chromosome 12 subfamily had been characterized and studied so far. Establish of gene expression analysis of all individual PSA genes will be needed to explore the importance of these genes in the different stages of the life cycle of Leishmania. In this study, we examined the expression of all members of PSA family, in Procyclic Promastigotes and Metacyclic Promastigotes of L. major by RT-PCR, and identified the relative abundance of their transcripts in these stages using RT-qPCR. Our results demonstrated that all PSA genes were expressed in the both stages of the life cycle of L. major. However, the levels of their transcripts vary notably according the genes and the genes with weak mRNA levels lie on Chr12. Furthermore, when studying the mRNA abundances of these genes during the growth of promastigotes from logarithmic phase to stationary phase we show that some PSA mRNA are present at similar levels in both life cycle stages while, the mRNA abundance of other PSA genes were considerably increased.Chr12 genes sub family has more variance of mRNA levels during promastigotes development. This work opens the possibility of a further more focused investigation of the functional role of these PSA genes in the different stages of the life cycle of Leishmania

إشراف وإعداد

الإشراف

شادي سكرية, محمود قويدر

الإعداد

عبد الرحمن يوسف

الاقتباسات الببليوغرافية