In Vitro Evaluation of a Novel Computationally Designed Anticancer Peptide in Ovarian Cancer Cell Models

الموضوعات

علوم تطبيقية وتكنولوجية

الملخص

Ovarian cancer remains one of the leading causes of cancer-related mortality among women, highlighting the need for novel therapeutic agents with improved selectivity and reduced toxicity. Anticancer peptides (ACPs) have emerged as promising candidates due to their ability to selectively target cancer cells. In this study, the anticancer activity of a computationally designed ACP was evaluated in vitro using the SKOV-3 ovarian cancer cell line, with HEK cells employed as a non-cancerous control. Cell viability was assessed using the MTT assay, and cytotoxicity was further confirmed by the trypan blue exclusion method. Apoptosis and necrosis were analyzed using Annexin V-FITC/Propidium iodide staining. The effects of ACP treatment on cell migration and invasion were examined using wound-healing and Transwell invasion assays, respectively. Quantitative real-time PCR (qPCR) was performed to evaluate the expression of genes associated with apoptosis, proliferation, and epithelial–mesenchymal transition (EMT), with GAPDH used as a housekeeping gene. ACP treatment resulted in a dose-dependent reduction in SKOV-3 cell viability, while minimal cytotoxic effects were observed in HEK cells, indicating selective anticancer activity. Trypan blue analysis confirmed increased cell death following ACP treatment. Apoptosis analysis revealed a significant increase in early apoptotic cells in ACP-treated SKOV-3 cells compared with untreated controls. Functional assays demonstrated a marked inhibition of cell migration and invasion upon ACP treatment. Gene expression analysis showed modulation of apoptosis- and proliferation-associated markers; however, no statistically significant changes were observed in the EMT-related genes TWIST1 and N-cadherin. This study provides functional evidence that the computationally designed anticancer peptide (ACP) exhibits selective anticancer activity against ovarian cancer cells in vitro, associated with increased apoptosis and reduced migratory and invasive behaviors.

روابط وملفات

التعريف والنوع

رقم الوثيقة

0ec69490-54f4-454b-a426-05564e39acb3

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل ماجيستير

نوع الملف

pdf text

أسماء الملفات

1085175_2.pdf

بيانات النشر

ألقاب المؤلفين

[{"name_ar":" Asma Amleh","title_ar":"اشراف","title_en":"Supervision"},{"name_ar":"Basma Shaala","title_ar":"اعداد","title_en":"Preparation"}]

اللغة

English

المصدر والدورية

اسم المصدر

In Vitro Evaluation of a Novel Computationally Designed Anticancer Peptide in Ovarian Cancer Cell Models

المحتوى والصفحات

عدد الصفحات

0

كلمات الباحثين

Ovarian Cancer, Anticancer Peptides, Apoptosis, Cytotoxicity, Cell Migration, Computational Peptide Design.

إشراف وإعداد

الإشراف

Asma Amleh

الإعداد

Basma Shaala

الاقتباسات الببليوغرافية