Effect of Nutrient Medium Strength Cytokinins and Auxins on Micropropagation of Gardenia (Gardenia jasminoides L.) using Nodal Explants

الموضوعات

زراعة

الملخص

Gardenia is one of the most important ornamental plants worldwide due to its beautiful flowers. Propagation of gardenia by conventional methods is slow and difficult. The main objective of this study was to develop a rapid propagation method using micropropagation techniques. Experiments were conducted to study the effect of different strengths (quarter, half, full) of Murashige and Skoog medium (MS) on gardenia morphogenesis. The effect of different concentrations (0.0, 0.5, 1.0, 2.0 and 3.0 mg/l) of both benzylaminopurine (BAP) and Isopentenyladenine (2ip) on shoot morphogenesis were also tested. The effect of different concentrations (0.0, 0.2, 0.4, 0.6, 0.8 and 1.0 mg/l) of indole 3-butyric acid (IBA), on quarter (MS) medium containing 2.0 mg/l BAP, on multiplication rate of gardenia using nodal explants were tested. Rooting of gardenia plantlets was investigated on different concentrations (0.0, 0.5, 1.0, 2.0 and 3.0 mg/l) of naphthalene acetic acid (NAA). The effect of different types of media ( silt, peat moss, silt and Peat moss 1:1, silt & sand 2:1 ) were tested for the acclimatization of the plantlets, All experiments were arranged in a completely randomized design. Results showed that the best medium strength for micropropagation of gardenia was quarter MS medium. Application of 2.0 mg/l BAP gave higher number of shoots (4.0) and shoot length (14.7 mm) while 0.5 mg/1 BAP gave higher shoot length (27.5 mm). Also, 2.0 mg/l 2ip gave 4.0 shoots/explant. Combination of 0.2 mg/l IBA with 2.0 mg / l BAP gave the highest number of shoots (5.8) and shoot length (8.4 mm). The highest number of roots (14) and root length (47.50 mm) were obtained on quarter MS medium containing 1.0 mg/l NAA. It is recommended to propagate gardenia using quarter MS supplemented with 2.0 mg/l BAP and 0.2 mg/l IBA while rooting initiation on quarter MS with 1.0 mg /l NAA. Silt: sand (2:1) was recommended for acclimatization of regenerated plants .

روابط وملفات

التعريف والنوع

رقم الوثيقة

0ec4e748-f6bc-4487-8058-70955c1613a0

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل ماجيستير

نوع الملف

pdf image

أسماء الملفات

0ec4e748-f6bc-4487-8058-70955c1613a0_1.pdf

بيانات النشر

ألقاب المؤلفين

[{"name_ar":" Mohamed Ahmad Ali","title_ar":"اشراف","title_en":"Supervision"},{"name_ar":"Amjad Suleiman Khader Elyan","title_ar":"اعداد","title_en":"Preparation"},{"name_ar":"Ibtisam Basheir Abdalla ","title_ar":"اشراف","title_en":"Supervision"}]

اللغة

English

المصدر والدورية

اسم المصدر

Effect of Nutrient Medium Strength Cytokinins and Auxins on Micropropagation of Gardenia (Gardenia jasminoides L.) using Nodal Explants

المحتوى والصفحات

عدد الصفحات

0

إشراف وإعداد

الإشراف

Mohamed Ahmad Ali, Ibtisam Basheir Abdalla

الإعداد

Amjad Suleiman Khader Elyan

الاقتباسات الببليوغرافية