Evaluating the Oxidative Stress in Patients with Diabetes Mellitus(type I and type II) and Studying Genetic Level of Glutathione-S-transferaseµ 1

الموضوعات

طب

الملخص

Diabetes mellitus is a group of metabolic diseases in which there is a high blood glucose level over a prolonged period. Symptoms of high blood sugar include frequent urination, increased thirst, and increased hunger. If left untreated, diabetes can cause many complications. Hyperglycemia in diabetes creates free radicals. These free radicals produce oxidative stress and thus debilitate the endogenous antioxidant defense system. The present study was conducted in (75) diabetic patients, (25) of them were treated with insulin, other (25) were using insulin and metformin, and the last (25) were on metformin and glibenclamide. The study also included (25) apparently healthy subjects who were taken as a control group. These groups were collected from Merjan Teaching Hospital from November 2013 to April 2014. The sera obtained from the blood of patients and control subjects were used to measure fasting blood sugar, glycosylated hemoglobin, total anti-oxidant concentration, total protein, and glutathione S-transferase activity. Also, the blood samples from study subjects were used to extract DNA for the glutathione S-transferase mu gene deletion. The result showed there is no significant difference among three treated groups in family history (p=0.846). In comparison with the control group, diabetic patients showed a significant increase in glycosylated hemoglobin and fasting blood sugar while there was a significant decrease in serum total protein (p ˂ 0.01), total anti-oxidant concentration (p ˂ 0.01), and glutathione S-transferase activity (p ˂ 0.01) when compared with the control group. LSD test shows that there was a significant difference between (insulin and metformin) group and (metformin and glibenclamide) in total serum protein (p ≤0.05). (Metformin and glibenclamide) group had less mean difference with the control group. Both the (insulin and metformin) and (metformin and glibenclamide) groups were highly and significantly decreased in the total antioxidant when compared with the control group (p ≤0.01) and no significant difference between them. Also, the results showed that the insulin group was significantly decreased against control (p= 0.036). LSD test shows that there was no significant difference in glutathione S-transferase activity in the three treated groups (p ≥ 0.05). At the gene level of the study, genotyping of glutathione S-transferase mu 1 gene by polymerase chain reaction technique was defined as GSTμ1 and GSTμ0 or deletion association to the presence and absence of the guanine nucleotide in the gene sequence. There was a statistical difference in the genotyping distribution, and the frequencies of GSTμ0 among study groups were 44% for insulin and metformin group, 68% for metformin and glibenclamide, 44% insulin, and 28% for the control healthy group. Conclusions raised from the present study involve that diabetes mellitus is associated with decreased total anti-oxidant capacity. The increase in oxidative stress may lead to damage β-cells, which may lead to a decrease in insulin secretion. Genetic polymorphism of glutathione S-transferase mu 1 gene may be considered as a risk factor for both types of diabetes mellitus.

روابط وملفات

التعريف والنوع

رقم الوثيقة

0ebc8859-a373-444f-93c6-c7c783c1413e

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل ماجيستير

نوع الملف

pdf text

أسماء الملفات

999101_1.pdf

بيانات النشر

ترجمة العنوان

تقييم الاكسدة في المرضى الذين يعانون من مرض السكري (النوع الأول والنوع الثاني) ودراسة المستوى الوراثي للكلوتاثيون-S-ترانسفيراز µ 1

ألقاب المؤلفين

[{"name_ar":"مؤيد عمران الغزالي","title_ar":"اشراف","title_en":"Supervision"},{"name_ar":"ميسم علي امين عوض","title_ar":"اعداد","title_en":"Preparation"},{"name_ar":"نسرين جلال البياتي","title_ar":"اشراف","title_en":"Supervision"}]

اللغة

English

المصدر والدورية

اسم المصدر

Evaluating the Oxidative Stress in Patients with Diabetes Mellitus(type I and type II) and Studying Genetic Level of Glutathione-S-transferaseµ 1

المحتوى والصفحات

عدد الصفحات

0

كلمات الباحثين

Diabetes mellitus, Hyperglycemia, Antioxidants, Glutathione S-transferase, Genetic analysis, Gene polymorphism, Insulin, Metformin, Glibenclamide

إشراف وإعداد

الإشراف

مؤيد عمران الغزالي, نسرين جلال البياتي

الإعداد

ميسم علي امين عوض

الاقتباسات الببليوغرافية