Contribution à l’étude phytochimique et à l’évaluation de quelques activités biologiques des extraits des graines de Nigella sativa L.

الموضوعات

علوم بحتة وطبيعية

روابط وملفات

التعريف والنوع

رقم الوثيقة

0e6606b0-6cfc-493c-9b0a-2a2b59c588cb

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

ماستر (LMD)

نوع الملف

pdf text

أسماء الملفات

2143675_1.pdf

بيانات النشر

ألقاب المؤلفين

[{"name_ar":"Amaria Belarbi ","title_ar":"اشراف","title_en":"Supervision"},{"name_ar":"Khadidja Benzaza ","title_ar":"اعداد","title_en":"Preparation"},{"name_ar":"Siham Cheriet ","title_ar":"اعداد","title_en":"Preparation"}]

اللغة

French

المصدر والدورية

اسم المصدر

Contribution à l’étude phytochimique et à l’évaluation de quelques activités biologiques des extraits des graines de Nigella sativa L.

المحتوى والصفحات

عدد الصفحات

0

ترجمة الملخص

Nigella is an aromatic plant widelyused in traditional medicine in Algeria, it presentsmany active principles known by theirtherapeutic properties.In the present study, methanolic and aqueous extracts of Nigella Sativa L. seeds werestudied to quantify their polyphenol and flavonoid contents using Folinciocalteu and AlCl3,respectively. In addition, the antioxidant activity is also tested by the DPPH radical reductionmethod. In addition, the antibacterial power of these extracts was studied by the solid-statediffusion method, on reference bacterialstrains.The qualitative analysis of theseextracts by the preliminary tests revealed the presenceof phenolic compounds, tannins and flavonoids; thisisconfirmed by a quantitativeanalysisbased on the dosage of the latter.The qualitative analysis of these three extracts by TLC revealed the presence ofnumerous constituents among which: gallicacid, quercetin and vanillin.Wetried on the analysis of these extracts which revealed the richness of the extract AQand METI and METII in phenolic compounds which confirms the results of the dosage ofpolyphenols by the method of Folin-Ciocalteu, where the contents are respectively: 2.35,0.058 and 0.82 μg EAG/mg and in flavonoidswith contents of 0.211, 1.29 and 0.322 μg/mgSimilarly, the quantitative evaluation of the antioxidant power of theseextracts, confirmsthatthe methanolic extract by maceration showed better DPPH-inhibitory antiradical activity withthe lowest IC50 (14.71 μg/ml) followed by the methanolic extract by soxhlet (IC50 33.82μg/ml) and the aqueous extract with IC50 (80.75 μg/ml).Regarding the antimicrobial activity, the methanolic and aqueous extracts of NigellaSativa L. seeds showed an appreciated antimicrobial effect by inhibiting the growth ofdifferent pathogenic strains (Pseudomonas aeruginosa and Staphylococcus aureus). While, noantibacterial effect was revealed in the strains (Klebsiella pneumoniae and Enterococcusfaecalis.All these results highlight the different therapeutic applications of this plant intraditional medicine and encourage the search for new natural molecules with antioxidant andantibacterial properties, with the aim of investing them in different industries: pharmaceutical,cosmetic, food, etc.

كلمات الباحثين

Nigella Sativa

إشراف وإعداد

الإشراف

Amaria Belarbi

الإعداد

Khadidja Benzaza , Siham Cheriet

الاقتباسات الببليوغرافية