Screening of Toxoplasma gondii Antibodies in Pregnant and Aborted Women attending Wad Medani Maternity Teaching Hospital and Um Algura Hospital using Latex Agglutination and Electro-chemiluminescence immunoassay (ECLIA)

الموضوعات

علوم بحتة وطبيعية

الملخص

Toxoplasmosis is an infection caused by an obligate intracellular parasite Toxoplasma gondii in final and intermediate host. Toxoplasma gondii is commonly associated with congenital infections that are not clinically apparent. The infection in the first trimester pregnancy may cause severe congenital anomalies or even foetal loss. In congenitally infected children can cause devastating effects including eye blindness, neurological impairment and mental retardation. The parasite distributed throughout the worldwide, in Sudan was 34.1% and in Gezira state was 41.7%. This study aimed to diagnosis of T.gondii in pregnant and aborted women by latex agglutination test and Cobas TORCH testing for IgG and IgM antibodies. Total 100 samples of venous blood were collected from pregnant and aborted women where the women participants in Wad Medani city were 37 while in Um Algura were 63, pregnant women were 74 while aborted women were 26 during November-December 2014. These samples were diagnosed using latex agglutination test for IgG antibodies and Electro-chemiluminescence immunoassay for both IgG and IgM. The results showed that a seropositivity of Toxoplasma IgG antibodies by latex agglutination test was 69% while was represented as 52.6% by Electro-chemiluminescence immunoassay. The Toxoplasma IgM seropositivity was (5.1%). There was significant difference between two methods with (P <0.0001). The sensitivity and specificity of latex agglutination test was (94%) and (57.8%) respectively with positive predictive value (71.2%) and negative predictive value (89.6%). The seroprevalence of Toxoplasma IgG antibodies showed (67.6%) in Wad Medan and (69.8%) in Um Algura by latex agglutination test while by Electro-chemiluminescence immunoassay was 43.2% and 54% in Wad Medani in Um Algura respectively. The seroprevalence of Toxoplasma IgM antibodies was (7.9%) and this present in Um Algura. The high prevalence noted among age group 26-35. There is little relation between the positive results with clinical symptoms; strong correlation with risk factors specially eating undercooked meat (71%), consumption of raw meat (68.1%), and contact with cats (52.1%). There was negative correlation between the seropositivity and parity number. The Electro-chemiluminescence immunoassay remain high specific and sensitive method in diagnosing of Toxoplasmosis, but a high coast of both apparatus and test reagents may prevent applicability in rural area, so the latex agglutination test may be useful in screening for disease due to simple applicability. The study recommended that the screening of Toxoplasma gondii should be done to all pregnant women to prevent disease progression, control cats, avoiding eating raw and undercooked meat and drinking filtrated water.

روابط وملفات

التعريف والنوع

رقم الوثيقة

0e656cad-571d-444e-82a0-35cee2b7d6f3

رقم العقد

0

نوع الوسائط

Crawler

نوع المحتوى

الرسائل العلمية

صيغة المصدر

رسائل ماجيستير

نوع الملف

pdf text

أسماء الملفات

72575_1.pdf

بيانات النشر

ألقاب المؤلفين

[{"name_ar":" Bakri Yousif Mohamed Nour ","title_ar":"اشراف","title_en":"Supervision"},{"name_ar":"Adam Dawoud Abakar ","title_ar":"اشراف","title_en":"Supervision"},{"name_ar":"Mohamed Yousuf Mohamed Elshiekh","title_ar":"اعداد","title_en":"Preparation"}]

اللغة

English

المصدر والدورية

اسم المصدر

Screening of Toxoplasma gondii Antibodies in Pregnant and Aborted Women attending Wad Medani Maternity Teaching Hospital and Um Algura Hospital using Latex Agglutination and Electro-chemiluminescence immunoassay (ECLIA)

المحتوى والصفحات

عدد الصفحات

0

إشراف وإعداد

الإشراف

Bakri Yousif Mohamed Nour , Adam Dawoud Abakar

الإعداد

Mohamed Yousuf Mohamed Elshiekh

الاقتباسات الببليوغرافية